The amount of impurities may also be estimated by Tate's method, which is performed by weighing 5 grammes of fat into a separating funnel, dissolving in ether, and allowing the whole to stand to enable the water to deposit. After six hours' rest the water is withdrawn, the tube of the separator carefully dried, and the ethereal solution filtered through a dried tared filter paper into a tared flask. Well wash the filter with ether, and carefully dry at 100 deg. C. The ether in the flask is recovered, and the flask dried until all ether is expelled, and its weight is constant. The amount of fat in the flask gives the quantity of actual fat in the sample taken; the loss represents the water and other impurities, and these latter may be obtained from the increase of weight of the filter paper. _Starch_ may be detected by the blue coloration it gives with iodine solution, and confirmed by microscopical examination, or it may be converted into glucose by inversion, and the glucose estimated by means of Fehling's solution. _Iodine Absorption._--This determination shows the amount of iodine absorbed by a fat or oil, and was devised by Huebl, the reagents required being as follows:-- (1) Solution of 25 grammes iodine in 500 c.c. absolute alcohol; (2) solution of 30 grammes mercuric chloride in 500 c.c. absolute alcohol, these two solutions being mixed together and allowed to stand at least twelve hours before use; (3) a freshly prepared 10 per cent. aqueous solution of potassium iodide; and (4) a N/10 solution of sodium thiosulphate, standardised just prior to use by titrating a weighed quantity of resublimed iodine dissolved in potassium iodide solution. In the actual determination, 0.2 to 0.5 gramme of fat or fatty acids is carefully weighed into a well-fitting stoppered 250 c.c. bottle, dissolved in 10 c.c. chloroform, and 25 c.c. of the Huebl reagent added, the stopper being then moistened with potassium iodide solution and placed firmly in the bottle, which is allowed to stand at rest in a dark place for four hours. A blank experiment is also performed, using the same quantities of chloroform and Huebl reagent, and allowing to stand for the same length of time. After the expiration of four hours 20 c.c. of 10 per cent. solution of potassium iodide and 150 c.c. water are added to the contents of the bottle, and the excess of iodine titrated with N/10 sodium thiosulphate solution, the whole being well agitated during the titration, which is finished with starch paste as indicator. The blank experiment is titrated in the same manner, and from the amount of thiosulphate required in the blank experiment is deducted the number of c.c. required by the unabsorbed iodine in the other bottle; this figure multiplied by the iodine equivalent of 1 c.c. of the thiosulphate solution and by 100, dividing the product by the weight of fat taken, gives the "Iodine Number".