also impregnated the liquid in B with some yeast taken from the
funnel of A. We then replaced the porcelain dish in which the
curved escape tube of A had been plunged, by a vessel filled with
mercury. The following is a description of two of these
comparative fermentations and the results they gave.
[Illustration with caption: Fig 2]
[Illustration with caption: Fig. 3]
The fermentable liquid was composed of yeast-water sweetened with
5 per cent, of sugar--candy; the ferment employed was
sacchormyces pastorianus.
The impregnation took place on January 20th. The flasks were
placed in an oven at 25 degrees (77 degrees F.).
FLASK A, WITHOUT AIR.
January 21st.--Fermentation commenced; a little frothy liquid
issued from the escape tube and covered the mercury.
The following days, fermentation was active. Examining the yeast
mixed with the froth that was expelled into the mercury by the
evolution of carbonic acid gas, we find that it was very fine,
young, and actively budding.
February 3rd.--Fermentation still continued, showing itself by a
number of little bubbles rising from the bottom of the liquid,
which had settled bright. The yeast was at the bottom in the form
of a deposit.
February 7th.--Fermentation still continued, but very languidly.
February 9th.--A very languid fermentation still went on,
discernible in little bubbles rising from the bottom of the
flask.
FLASK B, WITH AIR.
January 21st.--A sensible development of yeast.
The following days, fermentation was active, and there was an
abundant froth on the surface of the liquid.
February 1st.--All symptoms of fermentation had ceased.
As the fermentation in A would have continued a long time, being
so very languid, and as that in B had been finished for several
days, we brought to a close our two experiments on February 9th.
To do this we poured off the liquids in A and B, collecting the
yeasts on tared filters. Filtration was an easy matter, more
especially in the case of A. Examining the yeasts under the
microscope, immediately after decantation, we found that both of
them remained very pure. The yeast in A was in little clusters,
the globules of which were collected together, and appeared by
their well-defined borders to be ready for an easy revival in
contact with air.
As might have been expected, the liquid in flask B did not
contain the least trace of sugar; that in the flask A still
contained some, as was evident from the non-completion of
fermentation, but not more than 4.6 grammes (71 grains). Now, as
each flask originally contained three litres of liquid holding in
solution 5 per cent of sugar, it follows that 150 grammes (2,310
grains) of sugar had fermented in the flask B, and 145.4 grammes
(2,239.2 grains) in the flask A. The weights of yeast after
drying at 100 degrees C. (212 degrees F.) were--
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