As a consequence of these conclusions it should be an easy matter
to show, in the majority of living beings, the manifestation of
the phenomena of fermentation; for there are, probably, none in
which all chemical action entirely disappears, upon the sudden
cessation of life. One day, when we were expressing these views
in our laboratory, in the presence of M. Dumas, who seemed
inclined to admit their truth, we added: "We should like to make
a wager that if we were to plunge a bunch of grapes into carbonic
acid gas, there would be immediately produced alcohol and
carbonic acid gas, in consequence of a renewed action starting in
the interior cells of the grapes, in such a way that these cells
would assume the functions of yeast cells. We will make the
experiment, and when you come to-morrow--it was our good fortune
to have M. Dumas working in our laboratory at that time--we will
give you an account of the result." Our predictions were
realized. We then endeavoured to find, in the presence of M.
Dumas, who assisted us in our endeavour, cells of yeast in the
grapes; but it was quite impossible to discover any. [Footnote:
To determine the absence of cells of ferment in fruits that have
been immersed in carbonic acid gas, we must first of all
carefully raise the pellicle of the fruit, taking care that the
subjacent parenchyma does not touch the surface of the pellicle,
since the organized corpuscles existing on the exterior of the
fruit might introduce an error into our miscroscopical
observations. Experiments on grapes have given us an explanation
of a fact generally known, the cause of which, however, had
hitherto escaped our knowledge. We all know that the taste and
aroma of the vintage, that is, of the grapes stripped from the
bunches and thrown into tubs, where they get soaked in the juice
that issues from the wounded specimens, are very different from
the taste and aroma of an uninjured bunch. Now grapes that have
been immersed in an atmosphere of carbonic acid gas have exactly
the flavour and smell of the vintage; the reason is that, in the
vintage tub, the grapes are immediately surrounded by an
atmosphere of carbonic acid gas, and undergo, in consequence, the
fermentation peculiar to grapes that have been plunged into this
gas. These facts deserve to be studied from a practical point of
view. It would be interesting, for example, to learn what
difference there would be in the quality of two wines, the grapes
of which, in the once case, had been perfectly crushed, so as to
cause as great a separation of the cells of the parenchyma as
possible; in the other case, left, for the most part, whole, as
in the case in the ordinary vintage. The first wine would be
deprived of those fixed and fragrant principles produced by the
fermentation of which we have just spoken, when the grapes are
immersed in carbonic acid gas, by such a comparison as that which
we suggest we should be able to form a priori judgment on the
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