The possession of Samos completed the dominion of Darius over the coasts of Anatolia. It was of greater importance to get into his power the two straits which separate Europe from Asia--the Hellespont and the Bosphorus. If the Greek cities on the Asiatic side were subject, the cities and lands beyond were still to be conquered, and with the conquest of these the Persian empire would set foot in Europe. Perinthus, a colony of the recently-conquered Samos, Selymbria on the northern shore of the Propontis, and Byzantium on the Bosphorus, both colonies of Megara, recognised the dominion of Darius; in Byzantium, the most important of these cities, a tyrant, Ariston by name, soon took the lead.[252] The European shore of the Hellespont, the Thracian Chersonesus, had been for more than forty years under the rule of a princely family, which sprang from Attica. One of the oldest noble families in Attica, which had retired from the country before the usurpation of Pisistratus in 560 B.C.--the Philaidae, had established a principality there, by protecting and securing the Doloncian Thracians in the peninsula against their fellow-countrymen the Apsinthians, who dwelt at the mouth of the Hebrus. The position which the first of these princes, Miltiades II., thus obtained in the Hellespont, filled the city of Lampsacus, which lay opposite, on the Asiatic shore, with jealousy and anxiety for her trade; the question in dispute was the control of the busy strait. Lampsacus waged long and vigorous war against Miltiades and his nephew and successor Stesagoras. The latter was followed by his younger brother, Miltiades III. (about 518 B.C.), who had taken the reins of government tightly in hand. The forces of the little principality did not suffice to offer resistance to the Persians; and the walls of Sestos and Cardia were insufficient. We hear of no resistance, and Miltiades passed into the series of Persian vassal princes. In this way he was secured against Lampsacus and Sigeum also, where Pisistratus, in league with Polycrates of Samos, had placed his younger son Hegesistratus as prince about the year 533 B.C., who became a vassal of Persia when Cambyses demanded ships from the Greek cities, or after the fall of Polycrates, or certainly when Darius extended his sovereignty over Samos.