The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
_The Tubular Body_ is from eight to ten inches in length, to which is
added a draw-tube with an engraved millimetre scale. By extending the
draw-tube greater magnification is obtained, but since this is at the
cost of definition it should hardly ever be employed in the examination
of bacteria. _A Triple Nose-piece_ is doubtless a convenience, saving
time which is otherwise spent in replacing objectives of different
magnifying powers; there is also less risk of injuring them. _Focus_
should be obtained by means of a rack and pinion coarse adjustment,
together with the most approved kind of fine adjustment. The sliding
tube cannot be recommended, as the motion may be stiff, encouraging the
use of force, which in turn may result in the objective being brought
violently into contact with the specimen, thus doing injury to the lens
or damage to the preparation; or it may get too loose and readily slip
out of focus.
_The Stage_ should be flat and rigid, either rectangular or circular,
so long as it is sufficiently large to accommodate plate cultivation. A
removable mechanical stage is of great advantage for working with high
powers, as a motile bacterium can be constantly kept in view, while one
hand is engaged in working the fine adjustment; it may also be employed
as a finder, if engraved with a longitudinal and vertical scale, and
provided with a stop. The mechanical stage must be removable, so that
the stage proper may be free from any attachments when required for the
examination of cultures.
_Diaphragms._--The plan of using a series of separate discs of
different sizes should be avoided, as they are easily lost, and
bacteriological investigations may have to be made under conditions in
which it is difficult to replace them. A better plan is a revolving
plate with apertures of different sizes, but the most convenient form
is the _iris diaphragm_.
_The Sub-stage Condenser_ is as necessary in biological work as in
the objective--in fact, the condenser and the objective should be
considered as forming one piece of optical apparatus; the microscope
must be regarded as incomplete without it.
It is by the _sub-stage condenser_ that the rays of light are
concentrated at one point, or on one particular bacterium; for the best
definition it is essential that there should be mechanical arrangements
for accurately centring and focussing the condenser. All this will be
explained and enlarged upon under “Practical Optics.”
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Elsewhere in the archive
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account