The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Place a slip of ruled glass on the stage; and having turned the
eye-piece so that the lines on the two glasses are parallel, read off
the number of divisions in the eye-piece which cover one on the stage.
Repeat this process with different portions of the stage-micrometer,
and if there be a difference, take the mean. Suppose the hundredth of
an inch on the stage requires eighteen divisions in the eye-piece to
cover it; it is plain that an inch would require eighteen hundred,
and an object which occupied nine of these divisions would measure
the two-hundredth of an inch. Take the instance supposed, and let
the microscope be furnished with a draw-tube, marked on the side
with inches and tenths. By drawing this out a short distance, the
image of the stage micrometer will be expanded until one division is
covered by twenty in the eye-piece. These will then have the value
of two-thousandths of an inch, and the object which before measured
nine will then measure ten; which, divided by 2,000, gives the decimal
fraction ·005.
Enter in a table the length to which the tube is drawn out, and the
number of divisions on the eye-piece micrometer equivalent to an
inch on the stage; and any measurements afterwards taken with the
same micrometer and object-glass may, by a short process of mental
arithmetic, be reduced to the decimal parts of an inch, if not actually
observed in them.
In ascertaining the value of the micrometer with a deep objective, if
the hundredth of an inch on the stage occupies too much of the field,
then the two-hundredth or five-hundredth should be used and the number
of the divisions corresponding to that quantity be multiplied by two
hundred or five hundred, as the case may be.
The micrometer should not be fitted into too deep an eye-piece, as it
is essential to preserve good definition. A middle-power Kellner or
Huyghenian is frequently employed; at all events, use the eye-piece of
lower power rather than impair the image.
The eye-lens above the micrometer should not be of shorter focus than
three-quarters of an inch, even with high-power objectives.
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