The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The German glasses of to-day afford satisfactory evidence both of
skill and workmanship displayed in their production. Their cost is
greater, then, for the reason given, as will be seen on reference to
Continental catalogues. The dry series of objectives cost somewhat
less, a 1/2-inch (numerical aperture 0·30) can be had for £1 10s., and
a 1/6-inch (numerical aperture 0·65) for £2. On the other hand, the
apochromatic series rapidly increase in price as the numerical aperture
approaches the limit of numerical aperture 0·40. The best of Zeiss’s
series are the 12 mm. (1/2-inch) and the 3 mm. (1/8-inch), numerical
aperture 1·4, both of which possess the optical capacity assigned to
them. These objectives are undoubtedly the finest to be met with in
the workshop of any optician. Achromatic objectives of Continental
manufacture have been as much improved as those of English make by the
introduction of the newer varieties of glass, as already explained,
while a new nomenclature has sprung up in consequence. We now have
semi-apochromatic and parachromatic. The German opticians have followed
Zeiss’s lead, since almost the same series of objectives are given
in the catalogues of Leitz, Reichert, and Seibert, while the quality
of both dry and immersion objectives is found to be much the same.
The low price of Reichert’s immersion objectives should be noted, as
their performance is quite perfect. A 1/12-inch (numerical aperture
1·30) of Leitz’s, with which I have worked at _bacteria_, has given
me much satisfaction; supplied by Watson and Baker at £5. A 1/12-inch
dry objective by the same maker (numerical aperture 0·87) costs £3,
and a water immersion 1/12-inch (numerical aperture 1·10) £3 5s. Leitz
reminds me that it requires a good lens of from six to seven hundred
magnifying power for the examination of bacteria. For this reason he
has constructed a new form of lens, a 1/10-inch oil-immersion of 2·5
mm. focus, for the purpose of adding to the resources of bacteriology.
This lens necessarily has a lower magnification than his former
1/12-inch oil-lens, but as it is less costly to manufacture it is
sold at a smaller price. The before-mentioned 1/12-inch, with a No. 3
compensating eye-piece, gives a magnification of over seven hundred
or eight hundred diameters. To secure the best results in using the
higher powers of Leitz’s, from No. 5 upwards, a cover-glass of 0·17 mm.
in thickness should be used, and care taken to make the length of the
draw-tube equal to 170 mm. This length of tube should be adhered to
in the use of this optician’s oil-immersion lenses. If the microscope
be provided with a nose-piece, the draw-tube should be drawn out to
160 mm.; in its absence it should be set at 170 mm., a deviation of
10 mm. or more from the correct tube-length deteriorates from the
value of Leitz’s oil-immersion objectives as of other opticians. It is
suggested that the German apochromatic combination of three cemented
Public-domain text, read in full here on John Shaqi.
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