The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
It is not difficult, then, to perceive the importance of Amici’s
discovery as to the value of a drop of water inserted between the
object and the objective, and it now seems somewhat surprising it
should have been so long neglected by opticians, since it is at once
seen to diminish the reflection which takes place in the incidence of
oblique light. The film of water not only gives increased aperture,
but also greater cleanness and sharpness to the image. The film, then,
as already shown, collects the straying away of peripheral rays of
light, and sends them on to the eye-piece, and greatly assists in
rendering the image more perfect, and materially aids in the removal
of residuary secondary aberrations; while with air, or dry objectives,
a certain amount of aberration takes place, sufficient to affect the
pencils on their passage from the radiant to the medium of the front
lens, adding a considerable ratio to the total spherical aberration
with the objective, which, in the case of wide angles, increases
disproportionately from the axis outwards. This can only be corrected
by a rough method of balancing; that is, by introducing an excess of
opposite aberration in the posterior lens. An uncorrected residuum,
rapidly increasing with larger apertures, is then left, and this
appears in the image amplified by the total power of the objective, so
that with a non-homogeneous medium there is a maximum angular aperture
which cannot be surpassed without undergoing a perceptible loss of
definition, provided working distance is required. If we abolish the
anterior aberration for all colours, by an immersion fluid which is
equal to cover-glass in refractive and dispersive power, the difficulty
is at once overcome. If, for instance, we have an objective of 140° in
glass (= 1·25 N.A.) and water as the immersion fluid, the aberration in
front would affect a pencil of 140°. Substituting a homogeneous medium,
the same pencil, contracted to the equivalent angle in that medium
of 112°, will be admitted to the front lens without any aberration,
and may be made to emerge from the curved surface also without any
disturbing aberration, but contracted to an angle varying from 70° to
90°. The first considerable spherical aberration of the pencil then
occurs at the anterior surface of the _second_ lens, where the maximum
obliquity of the rays is already considerably diminished.
[Illustration: Fig. 119.]
[Illustration: Fig. 119_a_.]
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