The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
A reference to the above table will at once show that the nomenclature
of objectives expresses at once the initial magnifying powers, but as
makers have great difficulty in so calculating their formulæ so as to
obtain the _exact_ power, these figures must be taken as approximate.
Thus a 1/4-inch, which should magnify 40 diameters if true to its
description, might actually magnify a little more or less.
The magnifying powers of Zeiss’s and other apochromatic objectives
can be ascertained by dividing the focal length of the objective in
millimeters into 250 mm. (the distance of distinct vision), thus
Focus millimetres 24 16 12 4 3 2 1·5
Initial magnifying
power 10·5 15·5 21 63 83 125 167 diameters.
The total magnification, when any eye-piece is working in conjunction
with an objective, is ascertained by multiplying the initial power of
the objective by that of the eye-piece.
The above calculations are all for a 10-inch tube-length. Should,
however, a shorter or longer length of body be employed, the
magnification can at once be ascertained by a proportion sum. If the
magnification be 180 with 10-inch tube-length, what would it be with a
6-inch body--10 : 6 :: 180 = 108 diameters.
Abbe designed three different forms of eye-pieces: 1, the searcher
eye-piece; 2, the working eye-piece; and 3, the projecting eye-piece.
The _Searcher_ is a negative form of low power. The working is both
negative and positive, the positive form of which is constructed
on a newer principle; while the projection is chiefly intended
for microphotography, its field being small and its definition
superlatively sharp. These are severally explained among eye-pieces.
High-Power Objectives.
_Points of Importance for securing the best results with High-power
Objectives._--Always give to the body-tube of the microscope the
length for which the objective is corrected, 0·160 mm. for the short
continental tube, and 0·250 mm. for the English tube (10-inch). Employ
both dry and immersion objectives mounted for correction, commencing
with a numerical aperture of 0·75 (that is about 100° in air). If the
graduation is not given in thickness of cover-glass apply to the maker
to correct this omission.
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