The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The object of the correctional movement is to increase the maximum
aplanatic aperture of the condenser by separating the lenses. If
the back of a wide-angled objective be examined when an object is
illuminated by the full aperture of the condenser, the edge of the
flame being in focus, it will be noticed that the illuminated portion
of the back lens will be oval and pointed instead of circular. Also
that when the condenser is racked up, although the external shape of
the illuminated portion becomes more circular, two dark patches will
appear on either side of the centre, showing the operation of the
spherical aberration of the condenser. If under these circumstances
the lenses are separated by means of the collar adjustment, the black
spots will be closed up, and a circular and evenly-illuminated disc of
illumination of a larger size will result. The wheel of diaphragms,
or a series of graduated diaphragm discs to drop into a holder, is
intended for critical work; the diaphragm can always be recorded, and
the identical illuminating cone reproduced.
Hence we have a simple method of graduating apertures between any two
contiguous diaphragms; if, for example, we place the lever to the left,
so that the lens may be separated as far as possible, and use a No.
6 diaphragm, and if, on examining the object, it is thought that the
illuminating cone is not large enough, and if when No. 7 is turned
on it is found too much, we can go back to No. 6, and by turning the
lever 60° towards the right, closing the lenses and increasing the
power a little, we shall obtain an aperture somewhere between Nos. 6
and 7 diaphragm. Thus we can by means of the correction collar graduate
the aperture with the facility as with an iris, and we can record any
particular aperture with a degree of accuracy foreign to the iris. It
must be admitted, however, that the cone of light transmitted by the
condenser is a very small one.
Powell also supplies an apochromatic oil-immersion condenser, numerical
aperture 1·40, but without collar correction; Fig. 134 shows the
sliding tube lowered by arm A and cell B withdrawn for changing stops,
which can be done without altering the focus of the condenser. Fig.
134_a_ shows the cell B closed and raised by arm A close to the back
lens of optical combination. In Fig. 134_b_ six of the principal stops
are shown. Powell’s dry apochromatic condenser, of nearly 0·9 aplanatic
cone, is also very good; but the high price of all is a bar to their
more general use. The speciality of these is the conversion of axis
light into condensed oblique incident light by the refraction of the
condenser.
Public-domain text, read in full here on John Shaqi.
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