The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The objectives used by Dr. Woodward, throughout, were a 1/8-inch of
Wales’s (new series), and a 1/16-inch immersion, of Powell & Lealand’s,
especially produced for work with the camera. The magnification varied
from 800 to 3,000 diameters, a frustule of _Grammatophora Marina_
magnified 2,500, and a scale of podura, marked 3,000 in my collection,
are equal in definition to those taken by a high-angle 1/12-inch
oil-immersion. Pathological specimens taken with lower powers are
equally instructive, a section of epithelial cancer showing both nuclei
and cells with distinctness.
Dr. Maddox in 1864 was also experimentally engaged in the improvement
of the processes of photography for the purpose of promoting the work
of microscopists. His labours were attended with great success. To
him we are indebted for the gelatine dry-plate process, which gave
a remarkable impetus to photography in general. Dr. Maddox has, for
a period extending over forty years, diligently and successfully
cultivated and promoted micro-photography. Among other workers to whom
we are indebted for improvements in micro-photography I may mention
Wenham, Draper, Shadbolt, Highley, Koch, Sternberg, Pringle, Leitz, and
Pfeiffer.
Dr. Koch justly claims the credit of having extended the application
of micro-photography to the delineation of bacteria. A series of
instructive micro-photographs were published by him in 1877.
The importance of the camera has become more manifest as the work
of the bacteriologist has progressed. Koch strongly advocated
micro-photography on the ground that illustrations, especially of
bacteria, should be as true to nature as possible. Dr. Edgar Crookshank
holds the same opinion, and in support of his views we have numerous
illustrations of the bacteria given in his valuable “Text-book of
Bacteriology.” But he does not disguise the truth that there are
difficulties to be encountered, the first of which is owing to the
fact that the smallest and most interesting bacteria can only be made
visible in animal tissues by _staining_. This drawback has been very
nearly overcome by the use of eosin-collodion. With this medium, and
by shutting off portions of the spectrum by coloured glasses, Koch
succeeded in obtaining photographs of bacteria, which were stained
with blue and red aniline dyes. This method, however, introduced a
disturbing element of another kind. Owing to the longer exposure
required, the results were wanting in definition, attributable, it was
thought, to vibrations of the apparatus produced by passing traffic, or
by assistants moving about over the floor of the laboratory.
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