The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
_Apparatus and Material_ used in micro-photography have, from time to
time, been greatly varied by different workers, some preferring to use
the microscope in the vertical position with the camera superimposed
or fitted on the eye-piece of the microscope tube; others, again,
prefer that both the microscope and the camera should be arrayed
horizontally. In another form the ordinary microscope is dispensed with
and the objective stage and mirror are adapted to the front of the
camera, together with a suitable arrangement for holding the object.
Lastly, the camera is lain aside, and an operating-room rendered
impervious to light, takes its place, and the image is projected and
focussed upon a ground glass screen held in its place by a separate
support. This method has been made practical since the introduction
into microscopy by Zeiss of the _projection_ eye-piece. It is well
known that micro-photographs can be produced by employing these
projection eye-pieces, as well as for screen illustrations in the
lecture-room.
[Illustration: Fig. 172.--Swift’s Horizontal Apparatus.]
With regard to the position of the microscope and camera, the
horizontal affords greater stability than the vertical, and is on this
account to be preferred. The simplest apparatus consists of a camera
fixed upon a base board, four or five feet in length, upon which
the microscope can be clamped, and which also carries the lamp and
bull’s-eye lens (Fig. 172). This arrangement I have found economical
and useful. No more elaborate arrangement is actually necessary.
Sunlight is no doubt the best, but a good paraffin lamp is a handy and
available illuminant.
With the former, and rapid plates, a short exposure of three or four
seconds, even when high powers are used, is found sufficient; whereas,
with the paraffin lamp it will vary from three to ten minutes.
Walmsley gives the following table for exposures with the lamp:--
1-1/2-inch objective 3 to 45 seconds.
2/3-inch " 7 to 90 "
4/10-inch " 1/2 to 3 minutes.
1/5-inch " 2 to 7 "
1/10-inch " 4 to 10 "
For micro-photography the following practical rules must be observed.
The sub-stage condenser may be dispensed with when low powers are used,
as well as the mirror, and the lamp so placed that the image of the
flat of the flame appears accurately adjusted in the centre of the
field of the microscope. The bull’s-eye lens is so interposed, that
the image of the flame disappears, and the whole field becomes equally
illuminated with high powers; the sub-stage achromatic condenser must
be used, and a greater intensity of illumination is obtained by placing
the lamp-flame edgeways. It is advisable to begin the practice of
micro-photography with low powers, and a trial experiment should be
made with some well-known object as the blow-fly’s tongue.
Public-domain text, read in full here on John Shaqi.
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