The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
be obtained, as well as for recording microscopical images. That by
good lamp-light fair impressions of objects under extreme magnification
can be secured is encouraging, but the negatives produced by such
illumination seldom, if ever, possess the characteristics of a really
good sunlight negative, where the sharpest details are combined with an
exquisite softness and harmony of half-tones.
If the mirror of the microscope be of good size, it will only be
necessary to make an arm on which to support the removed mirror outside
some southerly exposed window, since it is desirable to have a greater
distance between the mirror and the stage than would be possible were
the mirror attached in its usual place. Where the microscope mirror
is too small to be satisfactorily used, a rectangular wood-framed
looking-glass is readily mounted, with the aid of a few strips of wood,
so as to turn about both axes.
The rays from the plane side of the mirror should pass through a
condensing lens (of 8-10-inch focus, if possible), so placed that
they are brought to a focus before reaching the plane of the object.
The exact position of the condensing lens is a matter of experience;
usually, however, the most favourable illumination is obtained at that
point where the field is brilliantly and _uniformly_ illuminated,
just before the rays form the image source of light; the nearer the
focus the less disturbance from diffraction rings. Ordinary objectives
will require the employment of monochromatic light--produced either
by a deep blue solution of ammonia-sulphate of copper, or by the
green glass screen--since the optical and actinic foci do not usually
perfectly coincide. Powers up to the 3/4-inch will require no further
condenser; with the 1/4 or 1/6-inch objectives, the low power (1 or
3/4-inch) serves with advantage as an achromatic condenser, when
attached to the sub-stage. The Abbe condenser, although so important
for fine microscopical investigation, is not adapted to photography
unless a very wide cone of light is desired, which, for the majority
of preparations, is some advantage; a low-power objective, used as a
condenser, is found to be more satisfactory than the Abbe with a small
diaphragm.[30]
The greatest delicacy in manipulation is necessary, as in working with
a 1/12-inch objective a turn too much of the fine adjustment will cause
the image to vanish. With fine preparations of bacteria it is not
easy to trace the image, and hence the advantage of commencing with
a well-marked object, as that of the fly’s tongue. The development
and fixation of the image must be proceeded with as in the ordinary
photographic process. In the text-books of photography full accounts
of failures will be found, their causes and prevention. Numerous
papers and suggestions for micro-photographic work will also be found
scattered throughout the “Journal of the Royal Microscopical Society.”
Public-domain text, read in full here on John Shaqi.
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