The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The thickness of the film of selenite determines the particular tint.
If, therefore, we use a film of irregular thickness, different colours
are presented by the different thicknesses. These facts admit of very
curious and beautiful illustration, when used under the object placed
on the stage of the microscope. The films employed should be mounted
between two glasses for protection. Some persons employ a large film,
mounted in this way between the plates of glass, with a raised edge,
to act as a stage for supporting the object, it is then called the
“selenite stage.” The best film for the microscope is that which
gives blue, and its complementary colour yellow. The late Mr. Darker
constructed a selenite stage for the purpose (Fig. 182). With this a
mixture of colours will be brought about, by superimposing three films,
one on the other. By slight variations in their positions, produced
by means of an endless-screw motion, all the colours of the spectrum
can be shown. When objects are thus exhibited, it should be borne in
mind that all negative tints, as they are termed, are diminished, and
all positive tints increased; the effect of which is to mask the true
character of the phenomena.
For a certain thickness of selenite the ellipse will become a circle,
and we have thus what is called _circularly-polarised_ light, which is
characterised by the property that rotation of the analyser produces
no change of intensity. Circularly-polarised light is not, however,
identical with ordinary light; for the interposition of an additional
thickness of selenite converts it into elliptically (or in a particular
case into plane) polarised light.
It is necessary, for the exhibition of colour in our experiments, that
the plate of selenite should be very thin, otherwise the retardation
of one component vibration as compared with the other will be greater
by several complete periods for violet than for red, so that the
ellipses will be identical for several different colours, and the total
non-suppressed light will be sensibly white in all positions of the
analyser.
Two thick plates may, however, be so combined as to produce the effect
of one thin plate. For example, two selenite plates of nearly equal
thickness may be laid one upon the other, so that the direction of
greatest elasticity in the one shall be parallel to that of least
elasticity in the other. The resultant effect in this case will be that
due to the difference of their thicknesses. Two plates so laid are said
to be _crossed_.
[Illustration: Fig. 183.--_Red_ is represented by perpendicular lines;
_Green_ by oblique.]
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