The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
It is probable that the conditions of snow formation are more complex
than might be imagined, familiar as we are with the conditions relating
to the crystallisation of water on the earth’s surface. A great
variety of animal, vegetable, and other substances possess a doubly
refracting or depolarising structure, as: a quill cut and laid out flat
on glass; the cornea of a sheep’s eye; skin, hair, a thin section of a
finger-nail; sections of bone, teeth, horn, silk, cotton, whalebone;
stems of plants containing silica or flint; barley, wheat, &c. The
larger-grained starches form splendid objects; _tous-les-mois_, the
largest, may be taken as a type of all others. This presents a black
cross, the arms of which meet at the hilum (Fig. 190). On rotating the
analyser, the black cross disappears, and at 90° is replaced by a white
cross; another, but much fainter, black cross is seen between the arms
of the white cross, no colour being perceptible. But if a thin plate of
selenite be interposed between the starch-grains and the polariser, a
series of delicate colours appear, all of which change on revolving the
analyser, becoming complementary at every quadrant of the circle. West
and East India arrow-root, sago, tapioca, and many other starch-grains,
present a similar appearance; but in proportion as the grains are
smaller, so are their markings and colourings less distinct.
[Illustration: Fig. 190.--Potato Starch, under Polarised Light.]
Molecular Rotation.
For the purpose of studying the various interesting phenomena of
molecular rotation, a few necessary pieces of apparatus must be added
to the microscope. First, an ordinary iron three-armed retort stand,
to the lower arm of which must be attached either a polarising prism
or a bundle of glass plates inclined at the polarising angle; in the
upper an analysing prism. The fluid to be examined should be contained
in a narrow glass tube about eight inches in height, and this must be
attached to the middle arm. If the prisms be crossed before inserting
a fluid possessing rotatory power, the light passing through the
analyser will be coloured. If a solution of sugar be employed, and
the light which passes through the second prism is seen to be red,
but on rotating the analyser towards the right the colour changes
to yellow, and passes through green to violet, it may be concluded
that the rotation is right-handed. If, on the contrary, the analyser
requires to be turned towards the left hand, we conclude that the
polarisation is left-handed. These phenomena are wholly distinct from
those accompanying the action of doubly refracting substances upon
plane polarised light. It is not easy to explain in a limited space the
course to be followed in ascertaining the amount of rotation produced
by different substances. Monochromatic light should be used. If we are
about to examine a sugar solution with the prisms crossed, the index
attached to the analyser must first be made to point to zero. The sugar
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