The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
_Cambridge Rocking Microtome._--This new pattern Cambridge Rocking
Microtome (Fig. 234) possesses advantages over other instruments in use
for cutting flat sections, and not parts of a cylindrical surface. The
tube containing the paraffin is 30 millimetres in internal diameter
instead of 20 millimetres, as in the earlier forms. The forward
movement is also increased, so that an object 12 millimetres long can
be cut throughout its whole length. It is provided with a dividing
arc for reading off the thickness of the section in thousandths of a
millimetre. The razor may be fixed either with its edge at right angles
to the direction of motion of the object, or diagonally, for giving a
slicing cut. The object can also be raised and fixed in position clear
of the razor.
This microtome has both steadiness and stiffness in its geometrical
arrangement and bearings, while the simplicity and efficiency of its
mechanism for advancing the section between each stroke of the razor is
remarkable. Although it may appear more complicated at first sight, it
is found not to be so when brought into use.
[Illustration: Fig. 235.--Cathcart’s Microtome.]
[Illustration: Fig. 235_a_.--Section Cutting Holder for Microtome.]
_Cathcart’s Freezing Microtome._--This is a convenient and useful
microtome for freezing purposes. Since its first introduction it has
been much improved. The clamping arrangements give steadiness, and the
principal screw is more effective; the freezing-plate is circular,
and the arrangements made for preventing the ether from reaching the
upper plate secures the object in view. This instrument can now be used
for embedding as well as freezing. The directions for freezing are as
follows:--
1. Place a few drops of mucilage (one part gum to three parts water) on
the zinc plate.
2. Take a piece of the tissue to be cut, of about a quarter of an inch
in thickness, and press it into the gum.
3. Fill the ether bottle with anhydrous methylated ether, and push the
spray points into their socket. All spirit must of course have been
previously removed by soaking for a night in water. The tissue should
afterwards be soaked in gum for a like time before being cut.
Work the spray bellows briskly until the gum begins to freeze; after
this work more gently. Be always careful to brush off the frozen vapour
which, in a moist atmosphere, may collect below the zinc plate. If the
ether should tend to collect in drops below the plate, work the bellows
slower.
5. Raise the tissue by turning the milled head, and cut by sliding the
knife along the glass plates.
6. After use, be careful to wipe the whole instrument clean.
7. Should the ether point become choked, clear by means of the fine
wire provided for the purpose.
8. The instrument is intended for use with methylated sulphuric ether.
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