The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
the leaf. The mycelium of a mould developed in a solution of Bismarck
brown was clearly stained after having been washed in water, whilst
it is known that the mycelium, which frequently forms in coloured
solutions, picro-carmine, hæmatoxylin, &c., remained perfectly
colourless. Other aniline colours injected under the skin of frogs
stained the connective tissue as deeply as did the Bismarck brown; but
the striæ of muscle remained colourless. We may conclude, then, that
Bismarck brown possesses the quality of colouring living protoplasm
both in plants and in animals.
Cutting, Grinding, and Mounting Hard Structures.
Take the femur of cat, or rabbit, remove as much of the muscle as
possible and macerate it in water until quite clean; on removal hang it
up to dry. With a fine saw make transverse and longitudinal sections.
File the section down until flat, and smooth. Take some Canada balsam,
place a piece on a square of glass and warm gently over a lamp until
the balsam is plastic enough to allow of the section being pressed
into it, and set it aside to consolidate. Take a hone (“Water-of-Ayr”
stone), moisten it with water, and rub one side of the section upon it
until quite smooth, then place the glass slip, with the section still
attached, into methylated spirit, and in a very short time the section
will be separated; wash it and remount it on the reverse side, and
proceed to rub it down on the hone until it appears to be thin enough
for mounting. Polish both sides on a polishing strop with Tripoli
powder, and mount in Canada balsam.
[Illustration: Fig. 244.--Small Lathe for cutting and polishing
Sections of Teeth.]
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