The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Although alkaline glycerine has been recommended for several purposes
in micro-technique, it is not so well known as it should be how
serviceable it is as an extempore mounting solution in vegetable
histology. The best mixture for general use is composed of glycerine 2
ozs., distilled water 1-1/2 oz., solution of potash, B.P., 1/2 oz. This
combines the refringent property of the glycerine with the clearing
action of the caustic potash, while the swelling action of the potash
is considerably diminished.
_Cutting Sections of Hard Woods._--The lathe and circular saw will be
found as useful for cutting sections of the harder kinds of woods,
as for bone structure. It may be necessary to subject the older and
consequently harder pieces of wood to the action of steam for a few
hours to soften them, and afterwards transfer them to methylated
spirit, before making an attempt to cut sections. But the more open
woods, of one, two, or three years’ growth, will show all that may
be required, and these can be cut by hand, or with the microtome, as
already described.
With a little practice the finest and thinnest possible slices may be
cut by hand. It is usual to take off the first slice to give a smooth
and even surface to the specimen. Then turn the screw to raise it a
little, sprinkle the surface with spirit and water, and cut with a
light hand. Remove the cut sections with a fine camel’s-hair brush
or a section lifter (Fig. 250) to a small vessel containing water,
when the thinnest will float on the surface, and remove to methylated
spirit and water, where they should remain until they can be mounted.
Sections of hard woods, and those containing gum-resins, or other
insoluble material, must first be kept in methylated spirit or alcohol,
and finally transferred to oil of cloves, to render them sufficiently
transparent for mounting in Canada balsam.
If the structure of an exogenous wood is required to be examined,
the sections should be made in at least three different ways: the
transverse, the longitudinal, and the oblique, or, as they are
sometimes called, the horizontal, vertical, and tangential, each of
which will exhibit different appearances, as seen in Fig. 245: _b_ is a
vertical section through the pith of a coniferous plant, and exhibits
the medullary rays known to the cabinet-maker as the silver grain;
_e_ is a magnified view of a part of the same; the woody fibres are
seen with their dots _l_, and the horizontal lines _k_ indicating the
medullary rays cut lengthwise; _c_ is a tangential section, and _f_ a
portion of the same; the medullary rays _m m_, and the woody fibres
with vertical slices of the dots, are shown. Instructive preparations
will be secured by cutting oblique sections of the stem. The sections
seen are made from the pine. All exogenous stems, however, exhibit
three different appearances, according to the direction in which the
section is made.
[Illustration: Fig. 245.--Sections of Wood.]
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