The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Collapsible tubes for containing Canada balsam and dammar.
Soft rags or old pocket handkerchiefs for removing cedar oil from
lenses and cover-glasses. Chamois leather for wiping lenses and
removing dust.
_Reagents_, _alcohol_, bergamot oil, _celloidin_, dissolved in equal
parts of ether and alcohol.
_Ebner’s_ solution. (See Appendix.)
_Formalin_, glycerine, gelatine, _Klebs’_ and _Kleinenberg’s_
solutions. (See Appendix.) The latter consisting of a watery solution
of picric acid 100 parts; strong sulphuric acid two parts; filter, and
add distilled water 300 parts.
_Muller_ fluid. (See Appendix.)
_Osmic acid_, a five per cent. solution.
_Paraffin_, _spermaceti_ and _xylol_, _acetic acid_, _hydrochloric
acid_, a one per cent. solution with _alcohol_.
_Ammonia_ liquid, _ether_, _picro-lithium carmine_, _potash solution_.
_Safranine_, concentrated alcoholic solution of, and a watery solution.
_Turpentine_, _vesuvin_, water distilled and sterilised.
Aqueous solutions of the several dyes may be kept in bottles ready for
use.
To both aqueous and alcoholic solutions a few drops of phenol, or a
crystal of thymol, should be added as a preservative. For the rapid
staining of cover-glass preparations, it is convenient to have the
most frequently used stains--fuchsine, methyl-violet, &c.--in bottles
provided with pipette stoppers.
_Clearing Agents._--Oils of cedar wood, cloves, origanum, aniline,
terebene, toluol and xylol, benzol and spirits of turpentine.
_Mounting Media._--Acetate of potash solution concentrated, benzole,
balsam, glycerine jelly, Fanant’s medium, dammar and mastic, Canada
balsam in xylol, Hollis’s glue, zinc white.
Cement for fixing small specimens temporarily to a glass slide. Remove
all traces of moisture, place upon it a drop or two of a medium
prepared as follows:--Dissolve over a water bath 15 grammes of white
lac in 100 grammes of absolute alcohol, decant off the clear liquid,
and stand it by for a while.
As the alcohol evaporates from the warmed surface of the glass slide a
hard transparent coating is left. This may be slightly softened at any
time by means of a drop of oil of lavender. After arranging the objects
the heat of a spirit-lamp will cause the oil to evaporate, leaving
them firmly attached. Objects may be mounted on cover-glasses in a
similar way. A resinous mounting medium may then be employed in the
usual manner. If glycerine or glycerine jelly is the mounting medium
employed, collodion diluted with two or three times its volume of oil
of lavender may be found preferable as the fixing agent. The section
should be placed in position before the preparation dries and the oil
is evaporated.
Methylated spirit is often so largely adulterated with rock-oil as
to render it unsuitable for technical purposes. Even to varnishes it
imparts a fluorescent appearance as it dries off.
[Illustration: Fig. 252.--Iron Box for holding Sterilised Instruments
and Glass Plates.]
Public-domain text, read in full here on John Shaqi.
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