The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
One of the most efficient forms of incubators introduced into the
bacteriological laboratory is that known as Hearson’s (Fig. 259).
This consists of a chamber surrounded by a water-jacket, with water
space below, to afford room for the pipe, L, which conveys the heated
products from the flame of the lamp, T, through the water and back
again to the lantern. A is the water-jacket surrounding the chamber
containing the cultures; O, the pipe through which the water supply is
admitted; N, the tap for employing the same; M, the overflow pipe; S,
the capsule in a case attached by a tube to the lower plate outside;
D, a lever pivoted on the left, carrying at its free end a damper, F,
which, when resting on the chimney, V, effectually closes it; P, a
screw for adjusting the damper when starting the apparatus; H, a lead
weight for bringing more pressure on the capsule; K, a thermometer, the
bulb of which is inside and the scale outside the chamber.
[Illustration: Fig. 259.--The Baird-Hearson Biological Incubator.]
The treated products of combustion move in the direction indicated
until the water and chamber are sufficiently heated to distend the
capsule. When this point is reached the wire between S and P is pushed
up by the capsule, and the lever causes the damper to rise more or
less off the chimney, V, and on examining the thermometer the inside
of the chamber is at length found to remain steadily at the required
temperature.
When the thermometer registers the desired temperature, the lead
weight must be damped to the lever by means of the milled-head screw
which goes through it. After having been once adjusted the heat in
the interior will remain constant, notwithstanding the utmost changes
of temperature occurring in these latitudes, nor will very great
alterations in the size of the lamp-flame seriously interfere with the
results. The milled-head screw, P, must be turned, after the first
adjustment, during the whole time that the incubator is in use. Observe
the temperature before opening the door; observations taken afterwards
are worthless.
Preparation of Nutrient Media--Separation, and Cultivation of Bacteria.
[Illustration: Fig. 260.--Plate Cultivation Showing Colonies.]
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