The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science — John Shaqi
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
When, then, the values in any given cases of the expression _n_ Sin
_u_ (which is known as the “numerical aperture”) has been ascertained,
the objectives are instantly compared as regards their aperture, and,
moreover, as 180° in air is equal to 1·0 (since _n_ = 1·0 and the
sine of half 180° = 1·0) we see, with equal readiness, whether the
aperture is smaller or larger than that corresponding to 180° in air.
Thus, suppose we desire to compare the apertures of three objectives,
one a dry objective, the second a water immersion, and the third an
oil immersion; these would be compared on the angular aperture view
as, say 74° air angle, 85° water angle, and 118° oil angle, so that
a calculation must be worked out to arrive at the actual relation
between them. Applying, however, the _numerical_[15] notation, which
gives ·60 for the dry objective, ·90 for the water immersion, and
1·30 for the oil immersion, their relative apertures are immediately
recognised, and it is seen, for instance, that the aperture of the
water immersion is somewhat less than that of a dry objective of 180°,
and that the aperture of the oil immersion exceeds that of the latter
by 30%.
The advantage of immersion, in comparison with dry objectives, becomes
at once apparent. Instead of consisting merely in a diminution of the
loss of light by reflection or increased working distance, it is seen
that a wide-angled immersion objective has a larger aperture than a dry
objective of maximum angle, so that for any of the purposes for which
aperture is essential an immersion must necessarily be preferred to a
dry objective.
That pencils of identical angular extension but in different media are
different physically, will cease to appear in any way paradoxical if we
recall the simple optical fact that rays, which in air are spread out
over the whole hemisphere, are in a medium of higher refractive index
such as oil _compressed_ into a cone of 82° round the perpendicular,
_i.e._, twice the critical angle. A cone exceeding twice the critical
angle of the medium will therefore embrace a _surplus_ of rays which do
not exist even in the hemisphere when the object is in air.
Public-domain text, read in full here on John Shaqi.
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