The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
The apertometer is an auxiliary piece of apparatus invented by Abbe,
for testing the fundamental properties of objectives and determining
their numerical and angular apertures. This accessory of the microscope
involves the same principles as that of Tolles, which the late Mr. J.
Mayall and myself brought to the notice of the Royal Microscopical
Society of London in 1876. Abbe’s apertometer (Fig. 39) consists of a
flat cylinder of glass, about three inches in diameter, and half an
inch thick, with a large chord cut off, so that the portion left is
somewhat more than a semicircle; the part where the segment is cut
is bevelled from above downwards, to an angle of 45°, and it will be
seen that there is a small disc with an aperture in it denoting the
centre of the semicircle. To use this instrument the microscope is
placed in a vertical position, and the apertometer is placed upon the
stage with its circular part to the front and the chord to the back.
Diffused light, either from the sun or lamp, is assumed to be in front
and on both sides. Suppose the lens to be measured is a dry one-quarter
inch; then with a one-inch eye-piece having a large field, the centre
disc, with its aperture on the apertometer, is brought into focus.
The eye-piece and the draw-tube are now removed, leaving the focal
arrangement undisturbed, and a lens supplied with the apertometer is
screwed into the end of the draw-tube. This lens, with the eye-piece
in the draw-tube, forms a low-power compound microscope. This is now
inserted into the body-tube, and the back lens of the objective whose
aperture we desire to measure is brought into focus. In the image of
the back lens will be seen stretched across, as it were, the image of
the circular part of the apertometer. It will appear as a bright band,
because the light which enters normally at the surface is reflected
by the bevelled part of the chord in a vertical direction, so that in
reality a fan of 180° in air is formed. There are two sliding screens
seen on either side of the figure of the apertometer; they slide on
the vertical circular portion of the instrument. The images of these
screens can be seen in the image of the bright bands. _These screens
should now be moved so that their edges just touch the periphery of the
back lens._ They act, as it were, as a diaphragm to cut the fan and
reduce it, so that its angle just equals the aperture of the objective
and no more.
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