The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical scienceHogg, Jabez
History
The Microscope. Its History, Construction, and Application 15th ed.: Being a familiar introduction to the use of the instrument, and the study of microscopical science
Hogg, Jabez
Microscopy; Natural history
Perhaps the most important improvement effected in this form of the
simple microscope was that ascribed to the celebrated Dr. Wollaston,
who devised a doublet of two plano-convex lenses having their focal
lengths, in the proportion of one to three, mounted with their convex
side directed towards the eye of the observer, and the lens of shorter
focal length next the object. The explanation given of the correction
thus effected in Dr. Wollaston’s doublet will be best understood
on reference to the annexed diagram, _l l′_, in Fig. 49, being the
object for a segment of the cornea of the eye, and _d d′_ the stop or
diaphragm. Now, it will be seen that each pencil of light proceeding
from _l l′_, the object, is rendered excentrical by the limiting
aperture or the diaphragm _d d_; consequently, they pass through the
lenses on opposite sides of their common axis _o p_; thus each becomes
affected by opposite errors, which to some extent balance and correct
each other. To take the pencil _l_, for instance, as it enters the eye
at _r b_; _r b_ is bent to the right at the first lens, and to the left
at the second; and as each bending alters the direction of the blue
ray more than the red, and as the blue ray falls nearer the margin of
the second lens, where the refraction is greater than that nearer the
centre, and compensates to some extent for the greater focal length of
the second lens, the blue rays will emerge very nearly parallel, and
colourless to the eye. At the same time, its spherical aberration has
been diminished, since the side of the pencil as it proceeds through
one lens passes nearer the axis, and in the other nearer the margin.
This must be taken to apply to pencils farthest from the centre of
the object. Central rays, it is obvious, would pass both lenses
symmetrically, the same portions of rays occupying nearly the same
relative places in both lenses. The blue ray would enter the second
lens nearer its axis than the red; and being thus less refracted than
the red by the second lens, some amount of compensation would take
place, differing in principle, and inferior in degree, to that which
is found in the excentrical pencils. In the intermediate spaces the
corrections are still more imperfect and uncertain; and this explains
the cause of aberrations which must of necessity exist even in the
best-made doublet. It is, however, infinitely superior to a single
lens, and will transmit a pencil of an angle of from 35° to 50°.
The next step towards improving the simple microscope was in relation
to the eye-piece, and was effected by Holland. It consisted in
substituting two lenses for the first in the doublet, and placing a
stop between them and the third. The first bending of the pencils
of light being effected by two lenses instead of one, produces less
spherical and chromatic aberration, which are more nearly balanced or
corrected at the second bending, and in the opposite direction, by the
third lens.
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