The modes of origin of lowest organisms: including a discussion of the experiments of M. PasteurBastian, H. Charlton
History
The modes of origin of lowest organisms: including a discussion of the experiments of M. Pasteur
Bastian, H. Charlton
Life -- Origin; Spontaneous generation
*No. XLVIII.--Ammonic Acetate Solution.*--On the tenth day the fluid
was still quite clear, and free from sediment.
*No. XLIX.--Ammonic Oxalate Solution.*--On the tenth day there was no
distinct opalescence of the fluid, but a well-marked whitish flocculent
deposit. On microscopical examination no _Bacteria_ were found in the
fluid, and the deposit was made up by an aggregation of blackish and
colourless granules, mixed with a few crystals and a very few _Torula_
cells--all being held together by a sort of mucoid matrix. In the midst
of this matter were found two or three very small, much branched,
mycelial tufts of a fungus-growth.
*No. L.--Ammonic Carbonate Solution.*--On the tenth day the fluid
showed a very faint opalescence, with a small amount of deposit, and
a partial non-coherent scum on the surface, which, on microscopical
examination, was found to be composed partly of amorphous granules, and
partly of minute _Bacteria_, mixed with small necklace-like organisms.
The fluid itself contained, in suspension, a few small and sluggish
_Bacteria_, with a minute _Torula_ cell here and there.
*No. LI.--Ammonic Tartrate Solution* after twenty-four hours showed
the faintest opalescence of the fluid; in forty-eight hours there was
a bluish-white turbidity, and in seventy-two hours the turbidity was
well marked. When examined microscopically the fluid was found to
contain multitudes of very active _Bacteria_. On the thirteenth day
the turbidity was not so well marked, though there was a very thin
pellicle on the surface, and also the dirty-looking crumpled remains of
another pellicle at the bottom, which, on examination, was found to be
composed of an aggregation of _Bacteria_. The pellicle on the surface
was very thin, and composed only of a single layer of _Bacteria_. In
the fluid itself many _Bacteria_ were seen, of medium size, and mostly
sluggish in movement, though a few of them exhibited very active
rotatory movements. No _Vibriones_, _Leptothrix_, or _Torulæ_, were
found.
*No. LII.--Ammonic Tartrate and Sodic Phosphate Solution* after
twenty-four hours showed the faintest opalescence; in forty-eight
hours there was a bluish-white turbidity, which, in seventy-two hours,
had become more marked. When examined microscopically multitudes of
_Bacteria_ were found whose movements were very sluggish. On the
thirteenth day there was a well-marked whitish turbidity, due to
_Bacteria_ and _Vibriones_, a slight amount of deposit, and a firm
pellicle which was found to be composed, almost wholly, of long
unjointed _Vibriones_ and unsegmented _Leptothrix_ filaments, all of
which, when separate, exhibited the most distinct eel-like movements,
accompanied by an actual progression from place to place.
_Ammoniacal Solutions, unboiled, and exposed to Air in a Corked Bottle,
after Inoculation with a Drop of Fluid containing living_ BACTERIA_and_
TORULÆ. (_Temp._ 60°–65° F.)
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