*No. LVII.--Ammonic Tartrate Solution* after sixty hours showed a slight sediment, with bluish flakes attached to sides of flask. In eighty-four hours there was a general bluish opalescence, and on microscopical examination the fluid was found to contain multitudes of _Bacteria_. *No. LVIII.--Ammonic Tartrate and Sodic Phosphate Solution.*--After sixty hours there was a slight general bluish opalescence. In eighty-four hours the general opalescence was not more marked, but there were many flake-like aggregations in the fluid, which on microscopical examination were found to be aggregations of _Bacteria_. _Ammoniacal Solutions boiled (at 212° F.), and exposed to Air in Flasks whose Open Necks were only loosely covered with Paper Caps: subsequent Inoculation. (Temp. 75°–85° F.)._ *No. LIX.--Ammonic Tartrate Solution.*--The fluid remained quite clear, and free from all trace of turbidity up to the ninth day, when it was inoculated with some living _Bacteria_. In fifty hours after the inoculation there was a very faint opalescence of the fluid, which, in another 24 hours, had become much more marked. On microscopical examination it was found to contain multitudes of _Bacteria_. *No. LX.--Ammonic Tartrate and Sodic Phosphate Solution.*--After four days the fluid was still quite clear. In seven days no trace of general turbidity, though there was a minute dirty-grey aggregation about 1/14″ in diameter at the bottom of the flask. On the sixteenth day the grey aggregation had very slightly increased in size, though the fluid above was still perfectly clear. The grey mass was removed by a small pipette, and, on microscopical examination, it was found to be composed of an aggregation of minute extraneous fibres, mixed with blackish particles and amorphous granular matter, in which were growing many _Torula_-cells in all stages of development, and also a minute mycelium composed of branched _Leptothrix_-like fibres.[73] The clear fluid was then inoculated with some living _Bacteria_, and the bulb of the flask was replaced in the warm bath. After fifty hours the solution showed a bluish turbidity, which, in thirty-six hours more, had increased to a well-marked whitish opacity, and when examined, the fluid was found to be swarming with active _Bacteria_. [73] A deposit of this kind is almost invariably found in such solutions after their fermentability has been lowered by previous boiling. Growth takes place very slowly in these cases, and also when similar boiled fluids are contained _in vacuo_. _Solutions of Ammonic Tartrate and Sodic Phosphate were heated, in their respective Flasks, for Fifteen Minutes to the Temperatures mentioned below. The Necks of the Flasks were afterwards loosely covered with Paper Caps, whilst the Bulbs were immersed in a Water-Bath kept at a Temperature of 75°–85° F._ *No. LXI.--Solution heated to* 149° F. *No. LXII.* " " " 158° F. *No. LXIII.* " " " 158° F.