The Nature of Animal LightHarvey, E. Newton (Edmund Newton)
Science
The Nature of Animal Light
Harvey, E. Newton (Edmund Newton)
Phosphorescence
It is, of course, obvious that when luciferin oxidizes, some oxidation
products must be formed. Most observers have assumed the oxidation
products of luciferin to be relatively simple and to represent a rather
complete breaking down of the luciferin molecule. Carbon dioxide was
mentioned by Phipson (1872) as being formed. We have just seen that no
carbon dioxide is formed during the oxidation of _Cypridina_ luciferin
and there is evidence that no fundamental change at all occurs. It is
for this reason that I have called the oxidation product of luciferin
_oxyluciferin_.[7] As we shall later see, the change luciferin
oxyluciferin is to be compared to the oxidation of colorless dyes
(leuco-compounds) to the colored dye. The chemical properties of
oxyluciferin are similar to those of luciferin and the oxyluciferin can
be readily reduced to luciferin again.
[7] It is unfortunate that Dubois (1918 b) has used the term
oxyluciferine in a quite different sense from the present use. He
regards oxyluciferine as a substance still capable of giving light by
autoöxidation, and represents the steps in luminescence as follows:
"Co-luciférase + preluciférine = luciférine.
Luciférase + luciférine = oxyluciférine.
Oxyluciférine + oxygéne = lumiérè."
I should represent them as follows:
Luciferin + oxygen ⇆ oxyluciferin.
The reaction proceeds to right with light production only in presence of
luciferase.
Finally, we have the fluorescent substance of _Pyrophorus_ and
fireflies, which Dubois first called _pyrophorin_, but later, adopting
McDermott's terminology, speaks of as _luciferesceine_. This Dubois
regards as a substance intensifying the light and modifying its color by
changing invisible into visible rays. As we have seen, this theory,
while attractive, will not stand the test of critical examination.
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