The Nature of Animal LightHarvey, E. Newton (Edmund Newton)
Science
The Nature of Animal Light
Harvey, E. Newton (Edmund Newton)
Phosphorescence
As the hæmoglobin does not lose this power on boiling, whereas
luciferase does, the analogy is far from perfect. Many oxygen carriers
are known, however, which may be destroyed on boiling their solutions,
namely, the peroxidases of plant juices. Esculin will not luminesce with
peroxidase and H_{2}O_{2}, but pyrogallol or gallic acid will. If one
mixes a test tube containing pyrogallol solution + H_{2}O_{2} with
potato or turnip juice or almost any plant extract, a yellowish
luminescence appears. The plant extract loses the power to cause such
luminescence on boiling and the peroxidase will not dialyze. It is, of
course, comparable to luciferase and acts on the thermostable,
dialyzable pyrogallol-H_{2}O_{2} mixture, which is comparable to
luciferin. Curiously enough, although many hydroxyphenol and
amino-phenol compounds can be oxidized by peroxidase and H_{2}O_{2},
only pyrogallol and gallic acid will oxidize with light production. Many
other oxidizers can take the place of the peroxidase. A list of these is
given on page 151. No other peroxide can take the place of H_{2}O_{2}
with peroxidases as oxidizers, but a few can replace H_{2}O_{2} with
other oxidizers. This is brought out in Table 7.
TABLE 7
_Peroxides Giving Light with Pyrogallol and Oxidizers_
Key to column headings:
[A]: Oxidizer. (Equal parts added to a mixture of M/100 pyrogallol and the
peroxide)
[B]: H_{2}O_{2} 3 per cent.
[C]: Benzoyl hydrogen peroxide (insoluble powder)
[D]: Ozonized turpentine (one drop)
[E]: Na_{2}O_{2} (powder)
[F]: BaO_{2} (powder)
[G]: MnO_{2} (insoluble powder)
[H]: PbO_{2} (insoluble powder)
[I]: K persulfate M/10
[J]: Na perborate M/20
[K]: K perchlorate M/10
[L]: Quinone (insoluble crystals)
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