Whatever is seen, however, in the frozen tissue is to be accepted as normal. 117. Two points must be determined before reliance can be placed on observations of tissues chemically acted on: First, we must prove that the forms now visible existed before the preparation--the chemical action merely unveiling them; secondly, we must estimate the part played by the elements which have been removed in order to make the rest visible. We know, for example, that the nucleus often exists in the cell, though an acid may be needed to make it visible. We also know that cells which during life are quite free from visible granules are distinctly granulated after death, even without external chemical action. Imagine the explanation of a steam-engine to be attempted by first taking it to pieces, and examining these pieces, with no account of the coals and steam which had previously been _removed_ in order to facilitate the examination. When we know the part played by coals and steam, we may disregard these items of the active machine. So when we know the part played by water, fat, amorphous substance, and plasmode, we may describe nerve-tissue without taking these into account. 118. “You have convinced me,” said Rasselas to Imlac, “that it is impossible to be a poet.” My readers may, perhaps, infer from this enumeration of the difficulties that a knowledge of the minute anatomy of the nervous system is impossible. Not so; but a knowledge of these difficulties should impress us with the necessity for a vigilant scepticism, and the search after new methods. If the difficulties are fairly faced, they may be finally overcome. What we must resign ourselves to at present is the conviction that our knowledge is not sufficiently accurate to be employed as a basis of deduction in the explanation of physiological and psychological processes.[139] 119. Having said so much, let me add that there are some positive materials, and these yearly receive additions. The organites are described with a general agreement as to their composition and structure--although there is much that is hypothetical even here. Neurine is known under two aspects: the amorphous and the figured. The figured, which is the better known, comprises cells of different kinds, fibres and fibrils. The amorphous, more generally called _Neuroglia_, or nerve-cement, is less understood, and is indeed by many authorities excluded altogether from the nerve-tissue proper, and relegated to the class of connective tissues. THE NERVE-CELL.