It has also been stated that each separate leaflet circumnutates. A pinna was cemented with shellac on the summit of a little stick driven firmly into the ground, immediately beneath a pair of leaflets, to the midribs of both of which excessively fine glass filaments were attached. This treatment did not injure the leaflets, for they went to sleep in the usual manner, and long retained their sensitiveness. the movements of one of them were traced during 49 h., as shown in Fig. 158. On the first day the leaflet sank down till 11.30 A.M., and then rose till late in the evening in a zigzag line, indicating circumnutation. On the second day, when more accustomed to its new state, it oscillated twice up and twice down during the 24 h. This plant was subjected to a rather low temperature, viz., 62°–64° F.; had it been kept warmer, no doubt the movements of the leaflet would have been much more rapid and complicated. It may be seen in the diagram that the ascending and descending lines do not coincide; but the large amount of lateral movement in the evening is the result of the leaflets bending towards the apex of the leaf when going to sleep. Another leaflet was casually observed, and found to be continually circumnutating during the same length of time. The circumnutation of the leaves is not destroyed by their being subjected to moderately long continued darkness; but the proper periodicity of their movements is lost. Some very young seedlings were kept during two days in the dark (temp. 57°–59° F.) except when the circumnutation of their stems was occasionally observed; and on the evening of the second day the leaflets did not fully and properly go to sleep. The pot was then placed for three days in a dark cupboard, under nearly the same temperature, and at the close of this period the leaflets showed no signs of sleeping, and were only slightly sensitive to a touch. On the following day the stem was cemented to a stick, and the movements of two leaves were traced on a vertical glass during 72 h. The plants were still kept in the dark, excepting that at each observation, which lasted 3 or 4 minutes, they were illuminated by two candles. On the third day the leaflets still exhibited a vestige of sensitiveness when forcibly pressed, but in the evening they showed no signs of sleep. Nevertheless, their petioles continued to circumnutate distinctly, although the proper order of their movements in relation to the day and night was wholly lost. Thus, one leaf descended during the first two nights (i.e. between 10 P.M. and 7 A.M. next morning) instead of ascending, and on the third night it moved chiefly in a lateral direction. The second leaf behaved in an equally abnormal manner, moving laterally during the first night, descending greatly during the second, and ascending to an unusual height during the third night.