The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
The eyes of the butterflies, and indeed of almost all insects, afford
materials for a study which is complete in itself. When examined with
a tolerably high power, instead of finding each eye with an unbroken
spherical surface, it is seen that many are composed of thousands of
hexagonal divisions, each being the outer surface of a separate portion
termed the _ocellus_. In others these divisions are square; but in
all there is a layer of dark pigment surrounding their lower parts.
The ocelli may be partly removed from the eye, which will show how
their tapering forms are arranged. But here we have to consider how to
place them in balsam for preservation. The eye being removed from the
insect, and the dark pigment removed by the use of a camel-hair pencil,
must be allowed to remain in turpentine at least for some days. The
turpentine should then be renewed and the eye well washed in it just
before it is to be mounted. It may then be set in balsam in the same
way as any other object;--but here a difficulty is met with. The eye
being spherical upon the surface required, must necessarily be “folded”
or broken in attempting to flatten it. This difficulty may be often
overcome by cutting a number of slits round the edges; but some object
to this mode of treatment, and where it is practicable it is much more
satisfactory to mount one in the natural rounded form and another
flat. Instead, however, of mounting the organ _whole_, four or five
slides may be procured from each of the larger ones, as those of the
dragon-fly, &c.
The _antennæ_ also are often mounted on separate slides, as being
better suited for higher powers and more minute examination than when
connected with the insect. These two projecting organs, issuing from
the head, are jointed, and moveable at will. They differ very much in
form amongst the various species, and are well worth the attention of
the microscopist. They are usually mounted with the head attached, and
perhaps they are more interesting when thus seen. Some few are very
opaque, to prepare which the following method has been advised:--
Bleach the antennæ by soaking in the following solution for a day or
two:--
Hydrochloric acid, 10 drops.
Chlorate of potash, ½ drachm.
Water, 1 oz.
This will render them transparent. Wash well, dry, and mount in Canada
balsam. Instead of the above, a weak solution of chloride of lime may
be used, by which means the nerves will be well shown. Many, however,
are rendered transparent enough by simply soaking in turpentine for
a longer or shorter time. Where the antennæ, however, are “plumose,”
or feather-like, extreme care is required in mounting, though the
difficulty is not so great as some seem to think. If they are first
dried with gentle pressure, and then subjected to the action of the
air-pump in a small quantity of turpentine until the air is thoroughly
expelled, they can be easily finished upon the slide, especially when
balsam and chloroform are used.
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