The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Insects supply us with another series of beautiful objects, viz.,
the _feet_.[D] These are sometimes simply dried and mounted without
any medium, as before mentioned; but most of them are rendered much
more fit for examination by using balsam in their preservation, as it
greatly increases their transparency. The smaller kinds may be dried
with gentle pressure betwixt blotting-paper, and then immersed for
some days in turpentine, without requiring the treatment with liquor
potassæ. This immersion will render them beautifully transparent, when
they may be mounted in balsam in the usual manner.
[D] See Mr. Hepworth’s interesting articles on the fly’s
foot in the second and third volumes of the _Microscopic
Journal_.
It is, however, sometimes found difficult to fix the feet when
_expanded_, in which state the interest of the object is greatly
increased. Mr. Ralph recommends the following mode:--“First wash
the feet, while the insect is yet alive, with spirits of wine; then
holding it by a pair of forceps close to the edge of a clean piece of
glass, the insect will lay hold of the upper surface by its foot, then
suddenly drop another small piece of glass over it, so as to retain
the foot expanded, and cut it off with a pair of scissors, tie up and
soak to get rid of air.” Mr. Hepworth says that he never found any
difficulty in expanding the foot on a drop of water or well-wetted
slide, and laying a thin glass cover over it, tying with thread,
drying, and immersing in turpentine.
The mouth, also, with its organs, is an interesting object in
many insects. That of the common fly is often made use of, and is
comparatively easy to prepare. By pressing the head, the tongue (as it
is commonly termed) will be forced to protrude, when it must be secured
by the same means as the foot, and may be subjected to the soaking
in turpentine, and mounted as usual. The honey-bee is, however, very
different in formation, and is well worth another slide; indeed, even
in insects of the same class, the differences are many and interesting.
Another worthy object of study is the _respiration_ of insects, which
is effected by tracheæ or hollow tubes, which generally run through the
body in one or more large trunks, branching out on every side. These
terminate at the surface in openings, which are termed _spiracles_, or
breathing organs. The _tracheæ_ often present the appearance of tubes
constructed by a twisted thread, somewhat resembling the spiral fibres
of some plants. These are very beautiful objects, and are generally
mounted in balsam, for which reason they are mentioned here; but as
they evidently belong to the “dissecting portion,” they will be fully
treated of in another place.
Public-domain text, read in full here on John Shaqi.
Reviews
Reviews
No reviews yet
Be the first to share your thoughts on this work.
Join the Discussion
Join the discussion
Sign in to leave a comment or review.
Sign InorCreate an account