The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
Most objects intended for the polariscope may be mounted in Canada
balsam; but there are some exceptions to this. Many of the salts are
soluble in this medium, or their forms so injured by it, that glycerine
or oil has to be used (see Chapter IV.); others must be left in the dry
form, as before mentioned; and some few it is impossible to preserve
unchanged for any length of time. _Crystals_, however, are amongst the
most beautiful and interesting subjects for polarisation; and it is
very probable that, by the aid of the polariscope, new and valuable
facts are yet to be made known. For one who finds pleasure in form
and colour, there is a field here which will only open wider upon him
as he advances; and instead of being in anywise a merely mechanical
occupation, it requires deep and careful study. The little here said on
the subject will show this in some degree.
With almost every salt the method of _crystallization_ must be modified
to obtain the best forms; I may even go further than this, and say that
it is possible to change these forms to such a degree that the eye
can perceive no relationship to exist betwixt them. If a solution of
sulphate of iron is made, a small quantity spread evenly upon a slide,
and then suffered to dry whilst in a flat position, the crystals often
resemble the fronds of the common fern in shape. But if, whilst the
liquid is evaporating, it is kept in motion by stirring with a thin
glass rod, the crystals form separately, each rhombic prism having its
angles well defined, and giving beautiful colours with the polarized
light. Again, pyro-gallic acid, when allowed to flow evenly over the
slide in a saturated solution, covers the surface in long “needles,”
which are richly coloured by polarized light; but if any small portion
of dust or other matter should form a nucleus around which these
“needles” may gather, the beauty is wonderfully increased. A form very
closely resembling the “eye” of the peacock’s tail, both in form and
colour, is then produced, which to one uninitiated in crystallography
bears very little resemblance to the original crystal. From these
simple facts it will be clearly seen that in this, as in every other
department, study and experience are needful to give the best results.
To obtain anything like uniformity in the formation of crystals upon
the glass slide, every trace of grease must be removed by cleaning with
liquor potassæ or ammonia immediately before using, care also being
taken that none of the agent is left upon the slide, otherwise it may
interrupt and change their relative position, and even their form.
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