The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
CHLORIDE OF ZINC SOLUTION.--In the “Micrographic Dictionary” this is
stated to be “perhaps the best preservative known for animal tissues.”
Persons of great experience, however, have given a very different
opinion; but it is certainly very useful in many cases where a small
degree of coagulating action is not injurious. It is used of strengths
varying according to the softness of the parts to be preserved; the
average being 20 grains of the fused chloride to 1 oz. of distilled
water. To keep this liquid a lump of camphor may be left floating in
the bottle. I have heard complaints that this mixture becomes turbid
with keeping, but I think this must only be the case when some impurity
has got into the bottle.
CARBOLIC ACID.--This substance has not been known long enough to
warrant any decided opinion upon its merits. A solution of 1 part of
acid to 20 of water has been recommended on account of its antiseptic
properties.
CASTOR OIL.--This is a very useful preservative for crystals and
other objects. Many salts are quite destroyed when Canada balsam is
used with them; but very few are acted upon by this oil. To use it,
it must be dropped in a sufficient quantity to cover the crystal or
object to be preserved with a thin coating of oil. It may be necessary
sometimes to spread it with a needle or other instrument. The thin
glass should then be carefully placed upon it, so that all air may
be excluded; and should any oil be forced out, owing to the quantity
used being too great, it must be removed with blotting paper. When
the edge of the thin glass cover and the surrounding parts of the
slide are as clean as possible, a coating of sealing-wax varnish or
liquid glue must be applied and allowed to dry. A second or even a
third coating may be required, but not before the previous cover is
quite dry. These varnishes, however, are very brittle, and it is much
safer, as a finish, to use one of the tougher cements--gold-size, for
instance--which will render it doubly secure.
The above are the principal liquids, &c., used for preserving objects
in cells. The different cells may be here mentioned; and it is
recommended that these should always be kept some time before use in
order that the cement may become perfectly dry; and care must be taken
that no cement be used on which the preservative liquid employed has
any action whatever.
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