The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
DEANE’S COMPOUND.--This is usually deemed about the best medium for
preserving Algæ, mosses, &c., and is thus prepared:--Soak 1 oz. of best
gelatine in 4 oz. of water until the gelatine becomes soft, when 5 oz.
of honey heated to boiling-point are added; boil the mixture, and when
it has cooled, but not enough to become stiff, add ½ oz. rectified
spirit with which 5 or 6 drops of creosote have been well mixed, and
filter the whole through fine flannel. This compound when cold forms a
stiff jelly, the use of which will be described elsewhere.
GLYCERINE JELLY.--This mixture closely resembles the above, but as the
composition differs a little it may be mentioned here. It is strongly
recommended by Mr. Lawrance in the _Microscopic Journal_, where he
states “that the beautiful green of some mosses mounted two years
ago, is still as fresh as the day they were gathered;” and that this
is the only medium he knows which will preserve the natural colour of
vegetable substances. He takes a quantity of Nelson’s gelatine, soaks
it for two or three hours in cold water, pours off the superfluous
water, and heats the soaked gelatine until melted. To each fluid ounce
of the gelatine, _whilst it is fluid but cool_, he adds a fluid drachm
of the white of an egg. He then boils this until the albumen coagulates
and the gelatine is quite clear, when it is to be filtered through fine
flannel, and to each ounce of the clarified solution add 6 drachms of a
mixture composed one part of glycerine to two parts of camphor-water.
GOADBY’S FLUID.--This is much used in the preservation of animal
objects; and seldom, if ever, acts upon the colours. It is thus
prepared--Bay salt, 4 oz.; alum, 2 oz.; corrosive-sublimate, 4 grains.
Dissolve these in two quarts of boiling water and filter. For delicate
preparations some recommend that this mixture be reduced by the
addition of an equal quantity of water; but where there is bone or
shell in the object the above acts injuriously upon it, in which case
this fluid may be used:--Bay-salt 8 oz., corrosive sublimate 2 grains,
water 1 quart.
THWAITES’ LIQUID.--This is recommended for the preservation of
Algæ, &c., as having little or no action on the colour, and is thus
prepared:--Take one part of rectified spirit, add drops of creosote
enough to saturate it; to this add sixteen parts of distilled water and
a little prepared chalk, and filter. When filtered, mix with an equal
quantity of camphor-water (as before mentioned), and strain through
fine muslin before using.
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