The preparation & mounting of microscopic objectsDavies, Thomas
Science
The preparation & mounting of microscopic objects
Davies, Thomas
Microscopy -- Technique
My friend, Mr. Rylands, successfully mounts the diatoms in the state
in which he finds them, and gave me the following method as that which
he always employs. He says that he has had no failures, and hitherto
has found his specimens unchanged. Take a shallow ring cell of asphalt
or black varnish (which must be at least three weeks old), and on the
cell, whilst revolving, add a ring of benzole and gold-size mixed in
equal proportions. In a minute or two pure distilled water is put in
the cell until the surface is slightly convex. The object having been
already floated on to the cover (the vessel used for this purpose being
an ordinary indian-ink palette), is now inverted and laid carefully
upon the water in the cell. By these means the object may be laid down
without being removed. The superfluous moisture must not be ejected by
pressure, but a wetted camel-hair pencil, the size made in an ordinary
quill, being partially dried by drawing through the lips, must be
used repeatedly to absorb it, which the pencil will draw by capillary
attraction as it is very slowly turned round. When the cover comes in
contact with the benzole and gold-size ring, there is no longer any
fear of the object being removed, and a slight pressure with the end
of the cedar stick of the pencil will render the adhesion complete,
and cement the cover closely and firmly to the cell. When dry, an outer
ring of asphalt makes the mounting neat and complete.
The _Fungi_ have been before mentioned; but it may be here stated that
some few of the minute forms are best preserved in a very shallow cell
of liquid. For this purpose creosote-water may be advantageously used.
The _antennæ_ of insects have been before noticed as being very
beautiful when mounted in balsam. This is readily accomplished when
they are large; but those of the most minute insects are much more
difficult to deal with, and are less liable to injury when put up in
fluid. _Goadby’s Fluid_ serves this purpose very well; but, of course,
the object must be thoroughly steeped in the liquid before it is
mounted, for a longer or shorter time according to the thickness.
The _eggs of insects_ afford some worthy objects for the microscope,
amongst which may be mentioned those of the common cabbage butterflies
(small and great), the meadow-brown, the puss-moth, the tortoiseshell
butterfly, the bug, the cow-dung fly, &c. These, however, shrivel up on
becoming dry, and must, therefore, be preserved in some of the fluids
before mentioned. To accomplish this no particular directions are
required; but the soaking in the liquid about to be employed, &c., must
be attended to as with other objects.
Public-domain text, read in full here on John Shaqi.
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