| | | | After one hour throat was| | | | again gargled. Count on | | | | blood agar | | | | Same gargle as above, but | 0 | 1 tablet| ... | 55,875,000 count was made on plain | | | | agar | | | | Normal conditions | 2 days | 0 | 79,125,000| ... One tablet was taken just | 1 hour | 1 tablet| ... | 56,250,000 ten minutes before gargle| 16 min. | | | was made | | | | Normal conditions | 2 days | 0 | 46,750,000| ... One tablet was taken just | 1 hour | 1 tablet| ... | 38,500,000 ten minutes before throat| | | | was gargled | | | | Teeth and mouth were thor-| 5 days | 0 | 47,370,000| ... oughly washed with soap | | | | just before gargle was | | | | made | | | | Teeth washed as above and | 1 hour | 1 tablet| ... | 21,225,000 1 tablet taken 10 minutes| | | | before gargle was made | | | | --------------------------+---------+---------+-----------+----------- Finally a determination was made of the number of streptococci in the throat before and after the use of Formamint. The throat was gargled in the manner previously described. The streptococcus count was made by the dilution method as given by Heinemann.[24] Culture tubes were used instead of fermentation tubes. One per cent. dextrose broth was the medium employed. One cubic centimeter was added to each of a series of ten tubes for each dilution and the following dilutions were used: 1:10,000, 1:100,000 and 1:1,000,000. [24] Heinemann: Laboratory Guide in Bacteriology, p. 86. The results given in Table 4 are the average count from a number of dilutions and are reported as the total number washed out by the 50 c.c. of salt solution. TABLE 4.--SHOWING THAT FORMAMINT FAILS TO REDUCE THE NUMBER OF STREPTOCOCCI IN THE THROAT