The Puering, Bating & Drenching of SkinsWood, Joseph Turney
Science
The Puering, Bating & Drenching of Skins
Wood, Joseph Turney
Leather
All three are bacteria to be found in the dog dung, and bacteria No.
3 consists of very small rods rounded at the ends and are exceedingly
mobile, while bacteria No. 7, which are similar to the hay bacillus,
are slowly-moving rods, and bacteria No. 12 are medium-sized rods
having rapid motion.
The prick-culture gives with bacteria No. 3 a very good growth in the
depth; from the inoculating prick extend laterally many arms into the
gelatin. At the ends of the arms are formed small knots. On the surface
is formed a thin white covering. The gelatin is not liquefied.
On gelatin plates, the deep-seated colonies consist of pale yellow
round disks, which gradually penetrate to the surface, and there they
form circular disks which show at the centre larger spots. On inclined
agar-agar there is formed a white superstratum.
With bacteria No. 7, the gelatin is strongly liquefied in the
prick-culture. On the surface of the zone of liquefaction there is
formed a white skin.
Along the liquefied prick are formed radiations into the solid gelatin.
On gelatin plates there are formed rapidly liquefying colonies forming
at top a white skin, agar-agar gives a white unequal thin superstratum
with branchings.
Bacteria No. 12 grow with prick-culture equally well along the prick;
the gelatin is not liquefied. On the surface there is formed a thin
shining covering.
On gelatin plates, the deep lying colonies consist of pale yellow
circular disks.
Many colonies have tails similar to the surface colonies of the
Proteus. During the growth of this bacterium on gelatin plates a strong
putrid smell is developed.
On agar-agar this bacterium forms a strong white shining superstratum.
These bacteria grow easily in any decoction of meat, and the flesh
particles from the flesh sides of the hides, which otherwise form a
waste product of little or no value, may be advantageously employed
for this purpose; bran, and other materials known to offer good
culture-beds, may be employed instead.
For this purpose a vessel provided with a cover is charged with equal
parts of waste flesh, such as is obtained in the tannery, and water,
and this is boiled for about half an hour by the direct introduction
of steam, after which concentrated carbonate of soda solution is
introduced until the reaction is slightly alkaline, after which the
decoction is cooled down. As soon as the temperature has sunk to about
35° C. there is added the pure culture obtained by the gelatin plate
process (with dilution), described in the works on bacteriology of
Koch, Fraenkel, Hueppe and others, and also by the further treatment
corresponding to the characteristic peculiarities of the bacteria, and
the whole is allowed to stand covered up for a period of from twelve
hours to five days, depending on the time of year and the temperature.
Public-domain text, read in full here on John Shaqi.
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