Fraction 1 was boiled with excess of barium carbonate, filtered, the BaCO_{3} washed with hot water, and the filtrate evaporated to dryness, and the Ba salts dried at 130° C. till the weight was constant; the salts were then decomposed with strong sulphuric acid, ignited, and the barium sulphate weighed. Fractions 2, 3, and 4 were treated in an exactly similar way, the barium salts obtained and the weight and percentage of barium sulphate being shown in the following table:-- ----------+-----------+-----------+----------- Fraction | Weight of | Weight of | Per Cent | Salts | BaSO_{4} | BaSO_{4} ----------+-----------+-----------+----------- I. | 0·5585 | 0·4991 | 89·365 II. | 0·5060 | 0·4618 | 91·265 III. | 0·31675 | 0·29415 | 92·86 IV. | 0·4475 | 0·4252 | 95·02 ----------+-----------+-----------+----------- Calculating the fractions 1 and 2 as mixtures of barium butyrate and acetate, and fractions 3 and 4 as mixtures of barium acetate and formate, we may summarise the results thus:-- ----------+-----------+---------+----------+--------- Fraction | Weight of | Ba | Ba | Ba | Salt | Acetate | Butyrate | Formate ----------+-----------+---------+----------+--------- I. | 0·5585 | 0·4904 | 0·0681 | .. II. | 0·5060 | 0·5030 | 0·0030 | .. III. | 0·31675 | 0·2749 | .. | 0·04185 IV. | 0·4475 | 0·2629 | .. | 0·18460 Total | 1·82875 | 1·5312 | 0·0711 | 0·22645 ----------+-----------+---------+----------+--------- or as free acids:-- ----------+--------+---------+-------- Fraction | Acetic | Butyric | Formic ----------+--------+---------+-------- I. | 0·2308 | 0·0385 | .. II. | 0·2367 | 0·0017 | .. III. | 0·1293 | .. | 0·0170 IV. | 0·1237 | .. | 0·0748 Total | 0·7205 | 0·0402 | 0·0918 ----------+--------+---------+-------- Proceeding now to (3) non-volatile bodies, we find these to consist of (1) acids, and (2) soluble carbohydrates. For examination, the remaining 4 litres of experimental drench were evaporated down to 1 litre and filtered; the residue (consisting of starchy matter) was well washed and the washings added to the filtrate, the whole placed in a large distilling-flask and distilled with continued addition of distilled water until the distillate was no longer acid (this required the addition and distillation of 4 litres of distilled water). A further deposit of solid matter rendering boiling dangerous, the liquid was again filtered and the residue washed free from acid. This residue was found to consist of nitrogenous organic matter and _calcium phosphate_, together with a trace of _calcium oxalate_, both being derived from the bran.