------+--------+--------+--------+--------- -- | Lactic | Formic | Acetic | Butyric ------+--------+--------+--------+--------- II. | 2·4380 | 0·5080 | 1·2083 | 0·1488 III. | 1·4737 | 0·3914 | 0·5593 | 0·0231 ------+--------+--------+--------+--------- [180] 2000 c.c. gave only about the same amount of acids as 1000 c.c. fermentations. For percentages of acids, see table, p. 281. FERMENTATION III., 2000 C.C. ---------+---------+---------+------------ |Weight of|Weight of|Per Cent. of Fraction| Salts | BaSO_{4}| BaSO_{4} ---------+---------+---------+------------ I. | 0·7260 | 0·6535 | 90·01 II. | 0·8150 | 0·7410 | 90·92 III. | 2·1525 | 2·1342 | 99·15 IV. | 0·3695 | 0·3627 | 98·16 V. | 0·3530 | 0·3500 | 99·15 ---------+---------+---------+------------ CALCULATION OF BARIUM SALTS AS BARIUM BUTYRATE, ACETATE, AND FORMATE. ---------+-----------+----------+----------- -- |Ba Butyrate|Ba Acetate|Ba Formate ---------+-----------+----------+----------- I. | 0·0597 | 0·6663 | .. II. | 0·0223 | 0·7927 | .. III. | .. | 0·6662 | 1·4863 IV. | .. | 0·1469 | 0·2226 V. | .. | 0·1070 | 0·2440 +-----------+----------+----------- Totals | 0·0820 | 2·3791 | 1·9529 ---------+-----------+----------+----------- Equivalent to 0·0463 grm. butyric acid. " 1·1186 " acetic " " 0·7828 " formic " or one-half of these quantities per litre of the fermented liquid. NOTE.--Mr. Adrian J. Brown, of Burton-on-Trent, has been kind enough to examine a sample of the glucose used in the above fermentations, and found the rotary power to be equivalent to 95·6 per cent. pure dextrose. The zinc salt of the lactic acid produced had no rotary power. *The Gases.*--In dealing with the gases evolved, we first compare those given off in the fermentation of glucose with that of bran under exactly similar conditions. The fermentation was conducted in open vessels as before described,[181] and the gases were collected and examined in the same way. [181] J.S.C.I. 1893, 423. MEAN OF THREE ANALYSES. -------+----------------+----------------- -- | Bran and Skins |Glucose and Skins -------+----------------+----------------- CO_{2} | 25·2 | 24·5 O_{2} | 2·1 | 1·5 H_{2} | 46·7 | 49·8 N_{2} | 26·0 | 24·2 -------+----------------+----------------- The composition of the gases is thus almost exactly similar, and, we think, fully proves our previous conclusions as to the change of the starch of the bran into glucoses by means of an unorganised ferment (cerealin).