Marine biology -- Great Britain; Seashore; Seashore biology
We have just referred to the hardening action of strong spirit as a
disadvantage, and so it is when it is required to preserve soft
structures with as little as possible of change in general form and
appearance; but there are times when it becomes necessary to harden
these soft structures in order that sections may be made for the purpose
of examining internal structure with or without the aid of the
microscope, and for such purposes strong spirit is one of the best
hardening agents that can be employed.
Formaldehyde is another very good preservative. It is a colourless
liquid, and should be considerably diluted for use, a two per cent.
solution being quite strong enough for all ordinary purposes. It
possesses some distinct advantages as compared with spirit. In the first
place, it does not destroy the natural colours of objects to the extent
that spirit does; and, although a hardening agent as well as a
preservative, it does not harden soft structures by the extraction of
the water they contain, and therefore does not cause them to become
shrivelled or otherwise distorted. It will also occur to the reader
that, since a small bulk of formaline represents a large volume of the
diluted preservative, it is very conveniently stored, and a very small
bottle of it taken for outdoor work may, on dilution with water, be made
to yield all that is required for the preservation of the takings of a
successful day, or even of a longer period. Formaldehyde is usually sold
in solution of about forty per cent. strength, and for the preparation
of a two per cent. solution it will be found convenient to provide a
glass measure graduated either into cubic centimetres or fluid ounces
and drams. One hundred volumes of the original solution contain forty of
pure formaldehyde, and if water be added to make this up to two thousand
volumes, a two per cent. solution is obtained. Thus, one hundred cubic
centimetres of the original solution is sufficient to prepare two litres
(three and a half pints) of suitable preservative.
A very good preservative liquid may be made by dissolving two ounces of
common salt, one ounce of alum, and two or three grains of corrosive
sublimate (a deadly poison) in one quart of water, and then, after
allowing all sedimentary matter to settle to the bottom, decanting off
the clear solution. This mixture is known as <i>Goadby’s fluid</i>, and is
well adapted for the preservation of both animal and vegetable
structures. It does not cause any undue contraction of soft tissues,
and, as a rule, does not destroy the natural colours of the objects kept
in it.
Glycerine is valuable as a preservative for both animal and vegetable
objects, and especially for the soft-bodied marine animals that form
such a large percentage of the fauna of our shores. It maintains the
tissues in a soft condition, and preserves the natural tints as well as
any liquid.
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