Further, although the turbulence of intervening atmosphere does
not interfere with the visibility of microscopic detail, a similar
disturbing factor does enter in the form of irregular and misplaced
illumination. It is a perfectly easy matter to make beautifully
distinct detail quite vanish from a microscopic image merely by
mismanagement of the illumination, just as unsteady atmosphere will
produce substantially the same effect in the telescopic image.
In the matter of magnification the two cases run quite parallel, and
magnification pushed beyond what is justified by the resolving power
of the instrument does substantially little or no good. It neither
discloses new detail nor does it bring out more sharply detail which
can be seen at all with a lower power.
The microscopist early learns to shun high power oculars, both from
their being less comfortable to work with, and from their failing to
add to the efficiency of the instrument except in some rare cases
with objectives of very high resolving power. Furthermore in the
interpretation of detail the lessons to be learned from the two
instruments are quite the same, although one belongs to the infinitely
little and the other to the infinitely great.
Nothing is more instructive in grasping the relation between resolving
power, magnification, and the verity of detail, than the study under
the microscope of some well known objects. For example, in Fig. 189
is shown a rough sketch of a common diatom, _Navicula Lyra_. The
tiny siliceous valve appears thus under an objective of slightly
insufficient resolving power. The general form of the object is
clearly perceived, as well as the central markings, standing boldly out
in the form which suggests the specific name. No trace of any finer
detail appears and no amount of dexterity in arranging the illumination
or increase of magnifying power will show any more than here appears,
the drawing being one actually made with the camera lucida, using an
objective of numerical aperture just too small to resolve the details
of the diatoms on this particular slide.
Figure 189_a_ shows what happens when, with the same magnifying power,
an objective of slightly greater aperture is employed. Here the whole
surface of the valve is marked with fine striations, beautifully
sharp and distinct like the lines of a steel engraving. There is a
complete change of aspect wrought by an increase of about 20% in the
resolving power. Again nothing further can be made out by an increase
of magnification, the only effect being to make the outlines a little
hazier and the view therefore somewhat less satisfactory.
[Illustration: FIG. 189.—The Stages of Resolution.]
Public-domain text, read in full here on John Shaqi.
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