II. Concerning the spontaneous production of the smallest microscopic animals it should be first observed, that the power of reproduction distinguishes organic being, whether vegetable or animal, from inanimate nature. The circulation of fluids in vessels may exist in hydraulic machines, but the power of reproduction belongs alone to life. This reproduction of plants and of animals is of two kinds, which may be termed solitary and sexual. The former of these, as in the reproduction of the buds of trees, and of the bulbs of tulips, and of the polypus, and aphis, appears to be the first or most simple mode of generation, as many of these organic beings afterwards acquire sexual organs, as the flowers of seedling trees, and of seedling tulips, and the autumnal progeny of the aphis. See Phytologia. Secondly, it should be observed, that by reproduction organic beings are gradually enlarged and improved; which may perhaps more rapidly and uniformly occur in the simplest modes of animated being; but occasionally also in the more complicated and perfect kinds. Thus the buds of a seedling tree, or the bulbs of seedling tulips, become larger and stronger in the second year than the first, and thus improve till they acquire flowers or sexes; and the aphis, I believe, increases in bulk to the eighth or ninth generation, and then produces a sexual progeny. Hence the existence of spontaneous vitality is only to be expected to be found in the simplest modes of animation, as the complex ones have been formed by many successive reproductions. _Experimental facts._ III. By the experiments of Buffon, Reaumur, Ellis, Ingenhouz, and others, microscopic animals are produced in three or four days, according to the warmth of the season, in the infusions of all vegetable or animal matter. One or more of these gentlemen put some boiling veal broth into a phial previously heated in the fire, and sealing it up hermetically or with melted wax, observed it to be replete with animalcules in three or four days. These microscopic animals are believed to possess a power of generating others like themselves by solitary reproduction without sex; and these gradually enlarging and improving for innumerable successive generations. Mr. Ellis in Phil. Transact. V. LIX. gives drawings of six kinds of animalcula infusoria, which increase by dividing across the middle into two distinct animals. Thus in paste composed of flour and water, which has been suffered to become acescent, the animalcules called eels, vibrio anguillula, are seen in great abundance; their motions are rapid and strong; they are viviparous, and produce at intervals a numerous progeny: animals similar to these are also found in vinegar; Naturalist's Miscellany by Shaw and Nodder, Vol. II. These eels were probably at first as minute as other microscopic animalcules; but by frequent, perhaps hourly reproduction, have gradually become the large animals above described, possessing wonderful strength and activity.