A great part of my work was done at the Zoological Station founded by Dr Dohrn at Naples; and I have to thank both Dr Dohrn and Dr Eisig for the uniformly obliging manner in which they have met my requirements for investigation. I have more recently been able to fill up a number of lacunæ in my observations by the study of embryos bred in the Brighton Aquarium; for these I am indebted to the liberality of Mr Lee and the directors of that institution. _The first appearance of the Spinal Nerves in Pristiurus._ In a _Pristiurus_-embryo, at the time when two visceral clefts become visible from the exterior (though there are as yet no openings from without into the throat), a transverse section through the dorsal region exhibits the following features (Pl. 22, fig. A):-- The external epiblast is formed of a single row of flattened elongated cells. Vertically above the neural canal the cells of this layer are more columnar, and form the rudiment of the primitively continuous dorsal fin. The neural canal (_nc_) is elliptical in section, and its walls are composed of oval cells two or three deep. The wall at the two sides is slightly thicker than at the ventral and dorsal ends, and the cells at the two ends are also smaller than elsewhere. A typical cell from the side walls of the canal is about 1/1900 inch in its longest diameter. The outlines of the cells are for the most part distinctly marked in the specimens hardened in either chromic or picric acid, but more difficult to see in those prepared with osmic acid; their protoplasm is clear, and in the interior of each is an oval nucleus very large in proportion to the size of its cell. The long diameter of a typical nucleus is about 1/3000 inch, or about two-thirds of that of the cell. The nuclei are granular, and very often contain several especially large and deeply stained granules; in other cases only one such is present, which may then be called a nucleolus. In sections there may be seen round the exterior of the neural tube a distinct hyaline membrane: this becomes stained of a brown colour with osmic acid, and purple or red with hæmatoxylin or carmine respectively. Whether it is to be looked upon as a distinct membrane differentiated from the outermost portion of the protoplasm of the cells, or as a layer of albumen coagulated by the reagents applied, I am unable to decide for certain. It makes its appearance at a very early period, long before that now being considered; and similar membranes are present around other organs as well as the neural tube. The membrane is at this stage perfectly continuous round the whole exterior of the neural tube _as well on the dorsal surface as on the ventral_.