Surrounding nearly the whole structure there is present a delicate investment similar to that which I mentioned as surrounding the neural canal and other organs; it is quite structureless, but becomes coloured with all staining reagents. I must again leave open the question whether it is to be looked upon as a layer of coagulated protoplasm or as a more definite structure. This investment completely surrounds the proximal portion of the posterior root, but vanishes near its distal extremity. The nerve-rudiment itself may be divided into three distinct portions:--(1) the proximal portion, in which is situated the pedicle of attachment to the wall of the neural canal; (2) an enlarged portion, which may conveniently, from its future fate, be called the ganglion; (3) a distal portion beyond this. The proximal portion presents a fairly uniform diameter, and ends dorsally in a rounded expansion; it is attached remarkably enough, not by its extremity, but by its side, to the spinal cord. The dorsal extremities of the posterior nerves are therefore free; as was before mentioned, they probably serve as the starting-point of the longitudinal commissures between the posterior roots. The spinal cord at this stage is still made up of fairly uniform cells, which do not differ in any important particulars from the cells which composed it during the last stage. The outer portion of the most peripheral layer of cells has already begun to be converted into the white matter. The delicate investment spoken of before still surrounds the whole spinal cord, except at the points of junction of the cord with the nerve-rudiments. Externally to this investment, and separated from it for the most part by a considerable interval, a mesoblastic sheath (Pl. 23, fig. H I, _i_) for the spinal cord is beginning to be formed. The attachment of the nerve-rudiments to the spinal cord, on account of its smallness, is still very difficult to observe. In many specimens where the nerve is visible a small prominence may be seen rising up from the spinal cord at a point corresponding to _x_ (Pl. 23, fig. H I). It is, however, rare to see this prominence and the nerve continuous with each other: as a rule they are separated by a slight space, and frequently one of the cells of the mesoblastic investment of the spinal cord is interposed between the two. In some especially favourable specimens, similar to the one figured, there can be seen a distinct cellular prominence (fig. H I, _x_) from the spinal cord, which becomes continuous with a small prominence on the lateral border of the nerve-rudiment near its free extremity. The absence of a junction between the two in a majority of sections is only what might be expected, considering how minute the junction is. Owing to the presence of the commissure connecting the posterior roots, some part of a nerve is present in every section.