Lining the two sides of the segmentation furrows there is present in sections a layer which stains deeply with colouring reagents; and the surface of the blastoderm is stained in the same manner. In neither case is it permissible to suppose that any membrane-like structure is present. In many cases a similar very delicate, but deeply-stained line, invests the vacuolar cavities, but the fluid filling these remains quite unstained. When distinct segments are formed, each of these is surrounded by a similarly stained line. The yolk-spherules are so numerous, and render even the thinnest section so opaque, that I have failed to make satisfactory observations on the behaviour of the nucleus. I find nuclei in many of the segments, though it is very difficult even to see them, and only in very favourable specimens can their structure be studied. In some cases, two of them lie one on each side of a furrow; and in one case at the extreme end of a furrow I could see two peculiar aggregations of yolk-spherules united by a band through which the furrow, had it been continued, would have passed. The connection (if any exists) between this appearance and the formation of the fresh nuclei in the segments, I have been unable to elucidate. The peculiar appearances attending the formation of fresh nuclei in connection with cell-division, which have recently been described by so many observers, have hitherto escaped my observation at this stage of the segmentation, though I shall describe them in a later stage. A nucleus of this stage is shewn on Pl. 6, fig. 6_c_. It is lobate in form and is divided by lines into areas in each of which a deeply-stained granule is situated. The succeeding stages of segmentation present from the surface no fresh features of great interest. The somewhat irregular (Pl. 6, figs. 4 and 5) circular line, which divides the peripheral larger from the central smaller segments, remains for a long time conspicuous. It appears to be the representative of the horizontal furrow which, in the Batrachian ovum, separates the smaller pigmented spheres from the larger spheres of the lower pole of the egg. As the segments become smaller and smaller, the distinction between the peripheral and the central segments becomes less and less marked; but it has not disappeared by the time that the segments become too small to be seen with the simple lens. When the spheres become smaller than in the germinal disc represented on Pl. 6, fig. 5, the features of segmentation can be more easily and more satisfactorily studied by means of sections. To the features presented in sections, both of the latter and of the earlier blastoderms, I now return. A section of one of the earlier germinal discs, of about the age of the one represented on Pl. 6, fig. 4, is shewn in Pl. 6, fig. 7.