Viewed from the surface (Pl. 8, fig. A) the blastoderm at this stage appears slightly oval, but the departure from the circular form is not very considerable. The long axis of the oval corresponds with what eventually becomes the long axis of the embryo. From the yolk the blastoderm is still well distinguished by its darker colour; and it is surrounded by a concentric ring of light-coloured yolk, the outer border of which shades insensibly into the normal yolk. At the embryonic portion of the blastoderm is a slight swelling, clearly shewn in Plate 8, fig. A, which can easily be detected in fresh and in hardened embryos. This swelling is to be looked upon as a local exaggeration of a slightly raised rim present around the whole circumference of the blastoderm. The roof of the segmentation cavity (fig. A, _s.c._) forms a second swelling; and in the fresh embryo this region appears of a darker colour than other parts of the blastoderm. It is difficult to determine the exact shape of the blastoderm, on account of the traction exercised upon it in opening the egg; and no reliance can be placed on the forms assumed by hardened blastoderms. This remark also applies to the sections of blastoderms of this stage. There can be no doubt that the minor individual variations exhibited by almost every specimen are produced in the course of manipulations while the objects are fresh. These variations may affect even the relative length of a particular region and certainly the curvature of it. The roof of the segmentation cavity is especially apt to be raised into a dome-like form. The main internal feature of this stage is the disappearance of the layer of cells which, during the first stage, formed the floor of the segmentation cavity. This disappearance is nevertheless not absolute, and it is doubtful whether there is any period in which the floor of the cavity is quite without cells. Dr Schultz supposes[120] that the entire segmentation cavity is, in the living animal, filled with a number of loose cells. Though it is not in my power absolutely to deny this, the point being one which cannot be satisfactorily investigated in sections, yet no evidence has come under my notice which would lead to the conclusion that more cells are present in the segmentation cavity than are represented on Pl. 13, fig. 1, of my preliminary paper[121], an illustration which is repeated on Pl. 7, fig. 2. Footnote 120: _Loc. cit._ Footnote 121: _Loc. cit._ The number of cells on the floor of the cavity differs considerably in different cases, but these cases come under the category of individual variations, and are not to be looked upon as indications of different states of development.