When the food-yolk becomes more bulky, and the hypoblast does not completely segment, it is not easy to understand why an ingrowth, which had no existence in the former case, should occur; nor where it is to come from. Such an ingrowth as is supposed to exist by Professor Haeckel would, in fact, break the continuity of development between meroblastic and holoblastic ova, and thus destroy one of the most important results of the Gastræa theory. It is quite easy to suppose, as I have done, that in the cases of discoidal segmentation, the hypoblast (including the yolk) becomes enclosed by the epiblast in precisely the same manner as in the cases of unequal segmentation. But even if Professor Haeckel supposes that in the unsegmented food-yolk a fresh element is added to the ovum, it remains quite unintelligible to me how an ingrowth of cells from a circumferential line, to form a layer which had no previous existence, can be equivalent to, or derived from, the invagination of a layer, which exists before the process of invagination begins, and which remains continuous throughout it. If Professor Haeckel's views should eventually turn out to be in accordance with the facts of vertebrate development, it will, in my opinion, be very difficult to reduce them into conformity with the Gastræa theory. Although some space has been devoted to an attempt to refute the views of Professor Haeckel on this question, I wish it to be clearly understood that my disagreement from his opinions concerns matters of detail only, and that I quite accept the Gastræa theory in its general bearings. * * * * * Observations upon the formation of the layers in Elasmobranchii have hitherto been very few in number. Those published in my preliminary account of these fishes are, I believe, the earliest[175]. Footnote 175: I omit all reference to a paper published in Russian by Prof. Kowalevsky. Being unable to translate it, and the illustrations being too meagre to be in themselves of much assistance, it has not been possible for me to make any use of it. Since then there has been published a short notice on the subject by Dr Alex. Schultz[176]. His observations in the main accord with my own. He apparently speaks of the nuclei of the yolk as cells, and also of the epiblast being more than one cell deep. In Torpedo alone, amongst the genera investigated by me, is the layer of epiblast, at about the age of the last described embryo, composed of more than a single row of cells. Footnote 176: _Centralblatt f. Med. Wiss._ No. 33, 1875. EXPLANATION OF PLATE 7. COMPLETE LIST OF REFERENCE LETTERS.