Intimately connected with the segmentation is the appearance and history of a number of nuclei which arise in the yolk surrounding the blastoderm. When the horizontal furrows appear which first separate the blastoderm from the yolk, the separation does not occur along the line of passage from the fine to the coarse yolk, but in the former at some distance from this line. The blastoderm thus rests upon a mass of finely granular material, from which, however, it is sharply separated. At this time there appear in this finely granular material a number of nuclei of a rather peculiar character. They vary immensely in size--from that of an ordinary nucleus to a size greater than the largest blastoderm-cell. In Pl. 3, fig. 1, _n_, is shewn their distribution in this finely granular matter and their variation in size. But whatever may be their size, they always possess the same characteristic structure. This is shewn in Pl. 3, figs. 1 and 2, _n_. They are rather irregular in shape, with a tendency when small to be roundish, and are divided by a number of lines into distinct areas, in each of which a nucleolus is to be seen. The lines dividing them into these areas have a tendency (in the smaller specimens) to radiate from the centre, as shewn in Pl. 3, fig. 1. These nuclei colour red with hematoxylin and carmine and brown with osmic acid, while the nucleoli or granules contained in the areas also colour _very intensely_ with all the three above-named reagents. With such a peculiar structure, in favourable specimens these nuclei are very easily recognised, and their distribution can be determined without difficulty. They are not present alone in the finely granular yolk, but also in the coarsely granular yolk adjoining it. They form very often a special row, sometimes still more markedly than in Pl. 3, fig. 1, along the floor of the segmentation cavity. They are not, however, found alone in the yolk. All the blastoderm-cells in the earlier stages possess precisely similar nuclei! From the appearance of the first nucleus in a segmentation-sphere till a comparatively late period in development, every nucleus which can be distinctly seen is found to be of this character. In Pl. 3, fig. 2, this is very distinctly shewn. (1) We have, then, nuclei of this very peculiar character scattered through the sub-germinal granular matter, and also universally present in the cells of the blastoderm. (2) These nuclei are distributed in a special manner under the floor of the segmentation cavity on which new cells are continually appearing. Putting these two facts together, there would be the strongest presumption that these nuclei do actually become the nuclei of cells which enter the blastoderm, and such is actually the case. In my account of the segmentation I have, indeed, already mentioned this, and I will return to it, but before doing so will enter more fully into the distribution of these nuclei in the yolk.