Several of these Crane-flies have passed through all their stages with me, and in nearly every case the transformation from pupa to perfect insect was made in water--in a tube three inches long, with a diameter of about an inch. The larvae were taken about the middle of September. My notes show that the first pupated on November 17, and the first fly came out on November 25. The long breathing-tube of the pupa was several times purposely displaced from its position on the surface. The creature was evidently incommoded, and twisted itself into strong curves; the head was thrown from side to side till part of the breathing-tube was raised above the surface and adhered to the side of the glass tube. Nor did the efforts cease till a considerable portion of the tube was in free communication with the air. This would seem to show that the air-supply is taken through the bladders which appear at irregular intervals in the breathing-tube, and not at the tip, where the keenest observers have failed to find an opening. INDEX Acridiidae, 80. Alder-fly, larva of, 177; pupa of, 182. American Cockroach, 74. Amphipods, 141; Rev. T. R. R. Stebbing on, 141. Aquaria, description of, 20. Arachnoidea, 96. _Argyroneta aquatica_, 108; De Geer on, 109; nest of, 110. Arthropods, 27; divisions of, 28; description of, 29. Ascidians, Darwin on, 13. _Asellus aquaticus_, description of, 153. Assassin Worm, 158. Aurivillius, Dr., on Crabs, 150. Bate and Westwood on Gammarus, 147; on Talitrus, 151. Bateman, Rev. G. C., on Hydrophilus, 50; on Nepa, 91; on China Marks, 171. Beakers, 21. Beetle Mite, 119. Belt on Blatta, 67. Black Beetle, 63. Black-bobs, 64. Blackwall on Spiders, 101, 102. _Blatta orientalis_, description of, 63, 66; Gilbert White on, 64; Dr. Sharp on, 65; Belt on, 67; Sir J. Lubbock on, 68; anatomy of, 68; breathing of, 70; Swammerdam on, 74; Professor Plateau on, 74. Book Scorpions, 96. _Brachelytra_, 59. Broad-clawed Porcelain Crab, 139. Brown China Mark, 172. Brushes, 23. Bugs, 86. Burgess, Mr., on larva of Dytiscus, 160. Butler, Dr., on Spiders, 100. Butler, Mr. E. A., on Forficula, 79. Capsules, 21. _Carcinus maenas_, description of, 136. Centipedes, 122; marine, 125. Cerci, 47. Chelate, meaning of word, 132 _n._ China Marks, 171; Rev. G. C. Bateman on, 171; De Geer on, 172. Cockroach, 63. Cocktail Beetle, 57. Coleoptera, 32. Collecting, method of, 25. Corixa, 94; Graber on, 95; Sir J. Lubbock on, 95. Crab, Shore, 136; Porcelain, 139. Crane-fly, 165. Crustacea, 128. Daddy-longlegs, 103. Dallas on Ocypus, 61.