It has been farther objected to experiments on animals with suspected articles of food, drink, or medicine, that it is difficult to administer poison to them in a state of concentration, and to prevent it from being discharged by vomiting. This objection, however, may be obviated by performing the experiment in the way recommended by Professor Orfila. A small opening is made into the gullet, previously detached from its surrounding connexions, the liquid part is introduced by a funnel thrust into the opening, and the solid portion previously made into little pellets is then squeezed down. Lastly, the gullet is tied under the aperture. The immediate effect of the operation is merely an appearance of languor; and no very serious symptom is observable till four or five days at soonest after the tying of the gullet. Hence if signs of poisoning commence within twenty-four hours, they are independent of the injury done by the operation.[109] This process requires some adroitness to execute it well. It cannot be tried successfully but by a practised operator, who, for reasons already given, would hardly ever try experiments of the kind with suspected articles. Mention is here made of it, therefore, chiefly because it is the best mode of experimenting in those cases in which it is necessary, as will presently be seen, to determine disputed points in the physiology of poisons. I may here shortly notice a method which has been lately proposed for detecting poisons that enter the blood, and which is founded on their effects on animals. M. Vernière suggests that advantage may be taken of the extreme sensibility of the medicinal leech to procure at least presumptive evidence, when no evidence can be procured in any other manner. He has related some experiments to prove that the leech, when placed in the blood of dogs killed by nux-vomica, is affected even when the quantity of the poison is exceedingly small.[110] It is extremely doubtful whether any importance can be attached to this criterion, as every one knows that the leech is apt to suffer from a variety of obscure causes, and among the rest from some diseased states of the body. 2. In the case of _the vomited matter_ or _contents of the stomach_ there are other and weightier objections to experiments on animals.—In the first place, the poison which has caused death may have been either in part or wholly vomited before-hand, or absorbed, or transmitted into the intestines, or decomposed by the process of digestion. Secondly, though abounding in the matter vomited or which remains in the stomach, it may be so much diluted, as not to have any effect on an animal. And, thirdly, the animal fluids secreted during disease are believed to act occasionally as poisons.